BD-PT4351-SNAI 1 Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

SNAI 1 Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PT4351

产品名称

SNAI 1 Rabbit Polyclonal Antibody

别名

SNAI1; SNAH; Zinc finger protein SNAI1; Protein snail homolog 1; Protein sna

类别

常规抗体

基因名称

SNAI1

蛋白名称

Zinc finger protein SNAI1(snail)

推荐应用

反应种属

Human,Mouse,Monkey

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

6615

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=6615

Human Swissprot No.

O95863

Human Swissprot Link

http://www.uniprot.org/uniprotkb/O95863/entry

Mouse Gene ID

20613

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=20613

Mouse Swissprot No.

Q02085

Mouse Swissprot Link

http://www.uniprot.org/uniprot/Q02085

免疫原

The antiserum was produced against synthesized peptide derived from human SNAI1. AA range:215-264

特异性

SNAI 1 Polyclonal Antibody detects endogenous levels of SNAI 1 protein.

稀释度

WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.

预测分子量

29kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

snail family transcriptional repressor 1(SNAI1) Homo sapiens The Drosophila embryonic protein snail is a zinc finger transcriptional repressor which downregulates the expression of ectodermal genes within the mesoderm. The nuclear protein encoded by this gene is structurally similar to the Drosophila snail protein, and is also thought to be critical for mesoderm formation in the developing embryo. At least two variants of a similar processed pseudogene have been found on chromosome 2. [provided by RefSeq, Jul 2008],

组织表达

Expressed in a variety of tissues with the highest expression in kidney. Expressed in mesenchymal and epithelial cell lines.

细胞定位

Nucleus . Cytoplasm . Once phosphorylated (probably on Ser-107, Ser-111, Ser-115 and Ser-119) it is exported from the nucleus to the cytoplasm where subsequent phosphorylation of the destruction motif and ubiquitination involving BTRC occurs. .

信号通路

Adherens_Junction;

功能

function:Seems to be involved in embryonic mesoderm formation. Binds to 3 E-boxes of the E-cadherin gene promoter and represses its transcription.,similarity:Belongs to the snail C2H2-type zinc-finger protein family.,similarity:Contains 4 C2H2-type zinc fingers.,tissue specificity:Expressed in a variety of tissues with the highest expression in kidney.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Western blot analysis of lysates from HT29 cells, using SNAI1 Antibody. The lane on the right is blocked with the synthesized peptide.

Immunofluorescence analysis of rat-heart tissue. 1,SNAI 1 Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of rat-kidney tissue. 1,SNAI 1 Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of rat-spleen tissue. 1,SNAI 1 Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Western Blot analysis of various cells using SNAI 1 Polyclonal Antibody diluted at 1：1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit （SC-003,Inventbiotech,MN,USA）.