产品名称
Smad6 Rabbit Polyclonal Antibody
别名
SMAD6; MADH6; Mothers against decapentaplegic homolog 6; MAD homolog 6; Mothers against DPP homolog 6; SMAD family member 6; SMAD 6; Smad6; hSMAD6
蛋白名称
Mothers against decapentaplegic homolog 6
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=4091
Human Swissprot No.
O43541
Human Swissprot Link
http://www.uniprot.org/uniprotkb/O43541/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=17130
Mouse Swissprot No.
O35182
Mouse Swissprot Link
http://www.uniprot.org/uniprot/O35182
免疫原
The antiserum was produced against synthesized peptide derived from human Smad6. AA range:323-372
特异性
Smad6 Polyclonal Antibody detects endogenous levels of Smad6 protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
The protein encoded by this gene belongs to the SMAD family of proteins, which are related to Drosophila 'mothers against decapentaplegic' (Mad) and C. elegans Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions in the negative regulation of BMP and TGF-beta/activin-signalling. Multiple transcript variants have been found for this gene.[provided by RefSeq, Sep 2014],
组织表达
Ubiquitous in various organs, with higher levels in lung. Isoform B is up-regulated in diseased heart tissue.
功能
function:Antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit selectively BMP (bone morphogenetic proteins) signaling by competing with the co-SMAD SMAD4 for receptor-activated SMAD1. SMAD6 is an inhibitory SMAD (I-SMAD) or antagonistic SMAD. Binds to regulatory elements in target promoter regions.,PTM:Phosphorylated by BMP type 1 receptor kinase and by PRKX.,similarity:Belongs to the dwarfin/SMAD family.,similarity:Contains 1 MH1 (MAD homology 1) domain.,similarity:Contains 1 MH2 (MAD homology 2) domain.,subunit:Interacts with NEDD4L (By similarity). Interacts with RNF111 and AXIN1. Interacts with TGF-beta type I receptor superfamily members, SMAD1, HOXC8 and HOXC9. Interacts with STAMBP and PRKX.,tissue specificity:Ubiquitous in various organs, with higher levels in lung. Isoform B is up-regulated in diseased heart tissue.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
