产品名称
Smad1/5/9 Rabbit Polyclonal Antibody
别名
SMAD1; BSP1; MADH1; MADR1; Mothers against decapentaplegic homolog 1; MAD homolog 1; Mothers against DPP homolog 1; JV4-1; Mad-related protein 1; SMAD family member 1; SMAD 1; Smad1; hSMAD1; Transforming growth factor-beta-signaling protein
蛋白名称
Mothers against decapentaplegic homolog 1/Mothers against decapentaplegic homolog 5/Mothers against decapentaplegic homolog 9
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene ID
4086/4090/4093
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=4086
Human Swissprot No.
Q15797/Q99717/O15198
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q15797/entry
Mouse Gene ID
17125/17129/55994
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=17125
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=59328
Rat Swissprot No.
P97588/Q9R1V3/O54835
Rat Swissprot Link
http://www.uniprot.org/uniprot/P97588
免疫原
The antiserum was produced against synthesized peptide derived from human Smad1/5/9. AA range:10-59
特异性
Smad1/5/9 Polyclonal Antibody detects endogenous levels of Smad1/5/9 protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-med
组织表达
Ubiquitous. Highest expression seen in the heart and skeletal muscle.
细胞定位
Cytoplasm . Nucleus . Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4 (PubMed:15647271). Co-localizes with LEMD3 at the nucleus inner membrane (PubMed:15647271). Exported from the nucleus to the cytoplasm when dephosphorylated (By similarity). .
功能
function:Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD).,PTM:Phosphorylated on serine by BMP type 1 receptor kinase.,PTM:Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.,similarity:Belongs to the dwarfin/SMAD family.,similarity:Contains 1 MH1 (MAD homology 1) domain.,similarity:Contains 1 MH2 (MAD homology 2) domain.,subcellular location:Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4.,subunit:Interacts with HGS, NANOG and ZCCHC12 (By similarity). May form trimers with another SMAD1 and the co-SMAD SMAD4. Interacts with PEBP2-alpha subunit, CREB-binding protein (CBP), p300, SMURF1, SMURF2 and HOXC8. Associates with ZNF423 or ZNF521 in response to BMP2 leading to activate transcription of BMP target genes. Interacts with LBXCOR1.,tissue specificity:Ubiquitous. Highest expression seen in the heart and skeletal muscle.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
