BD-PT4321

SLP-76 Rabbit Polyclonal Antibody

LCP2; Lymphocyte cytosolic protein 2; SH2 domain-containing leukocyte protein of 76 kDa; SLP-76 tyrosine phosphoprotein; SLP76

常规抗体

LCP2

Lymphocyte cytosolic protein 2

WB

Human,Mouse,Rat

1 mg/ml

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

3937

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=3937

Q13094

http://www.uniprot.org/uniprotkb/Q13094/entry

16822

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=16822

Q60787

http://www.uniprot.org/uniprot/Q60787

The antiserum was produced against synthesized peptide derived from human SLP-76. AA range:94-143

SLP-76 Polyclonal Antibody detects endogenous levels of SLP-76 protein.

WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200

75kD

-20°C/1 year

Polyclonal, Rabbit,IgG

SLP-76 was originally identified as a substrate of the ZAP-70 protein tyrosine kinase following T cell receptor (TCR) ligation in the leukemic T cell line Jurkat. The SLP-76 locus has been localized to human chromosome 5q33 and the gene structure has been partially characterized in mice. The human and murine cDNAs both encode 533 amino acid proteins that are 72% identical and comprised of three modular domains. The NH2-terminus contains an acidic region that includes a PEST domain and several tyrosine residues which are phosphorylated following TCR ligation. SLP-76 also contains a central proline-rich domain and a COOH-terminal SH2 domain. A number of additional proteins have been identified that associate with SLP-76 both constitutively and inducibly following receptor ligation, supporting the notion that SLP-76 functions as an adaptor or scaffold protein. Studies using SLP-76 deficient T c

Highly expressed in spleen, thymus and peripheral blood leukocytes. Highly expressed also in T-cell and monocytic cell lines, expressed at lower level in B-cell lines. Not detected in fibroblast or neuroblastoma cell lines.

Cytoplasm .

Natural killer cell mediated cytotoxicity;T_Cell_Receptor;Fc epsilon RI;

domain:The SH2 domain mediates interaction with SHB.,function:Involved in T-cell antigen receptor mediated signaling.,PTM:Phosphorylated after T-cell receptor activation by ZAP-70.,similarity:Contains 1 SAM (sterile alpha motif) domain.,similarity:Contains 1 SH2 domain.,subunit:Interacts with SLA. Interacts with CBLB (By similarity). Interacts with the adapter proteins GRB2 and FYB. Interacts with SHB. Interacts with PRAM1.,tissue specificity:Highly expressed in spleen, thymus, and peripheral blood leukocytes. Highly expressed also in T-cell and monocytic cell lines, expressed at lower level in B-cell lines. Not detected in fibroblast or neuroblasatoma cell lines.,

现货

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.