BD-PT4311-Skp2 p45 Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

Skp2 p45 Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PT4311

产品名称

Skp2 p45 Rabbit Polyclonal Antibody

别名

SKP2; FBXL1; S-phase kinase-associated protein 2; Cyclin-A/CDK2-associated protein p45; F-box protein Skp2; F-box/LRR-repeat protein 1; p45skp2

类别

常规抗体

基因名称

SKP2

蛋白名称

S-phase kinase-associated protein 2

推荐应用

反应种属

Human,Rat,Mouse

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

6502

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=6502

Human Swissprot No.

Q13309

Human Swissprot Link

http://www.uniprot.org/uniprotkb/Q13309/entry

Mouse Swissprot No.

Q9Z0Z3

Mouse Swissprot Link

http://www.uniprot.org/uniprot/Q9Z0Z3

免疫原

The antiserum was produced against synthesized peptide derived from human SKP2/p45. AA range:366-415

特异性

Skp2 p45 Polyclonal Antibody detects endogenous levels of Skp2 p45 protein.

稀释度

WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

预测分子量

47kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class; in addition to an F-box, this protein contains 10 tandem leucine-rich repeats. This protein is an essential element of the cyclin A-CDK2 S-phase kinase. It specifically recognizes phosphorylated cyclin-dependent kinase inhibitor 1B (CDKN1B, also referred to as p27 or KIP1) predominantly in S phase and int

组织表达

Epithelium,Liver,Placenta,Prostatic carcinoma,

细胞定位

Cytoplasm . Nucleus .

信号通路

Cell_Cycle_G1S;Cell_Cycle_G2M_DNA;Ubiquitin mediated proteolysis;Pathways in cancer;Small cell lung cancer;

功能

function:Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. Degradation of CDKN1B/p27kip also requires CKS1. Recognizes target proteins ORC1L, CDT1, RBL2, MLL, CDK9, RAG2, FOXO1A, UBP43, and probably MYC, TOB1 and TAL1. Degradation of TAL1 also requires STUB1. Recognizes CDKN1A in association with CCNE1 or CCNE2 and CDK2.,pathway:Protein modification; protein ubiquitination.,similarity:Contains 1 F-box domain.,similarity:Contains 8 LRR (leucine-rich) repeats.,subunit:Part of the SCF(SKP2) complex consisting of CUL1, RBX1, SKP1 and SKP2. Interacts directly with CUL1 and SKP1. Interacts with CKS1. Interacts with the cyclin A-CDK2 complex. Interacts with ORC1L, phosphorylated CDT1, phosphorylated RBL2, ELF4, phosphorylated RAG2, FOXO1A, UBP43, MYC, TOB1, TAL1 and MLL.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunofluorescence analysis of A549 cells, using SKP2/p45 Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using SKP2/p45 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from 293 cells, using SKP2/p45 Antibody. The lane on the right is blocked with the synthesized peptide.

Western Blot analysis of various cells using Skp2 p45 Polyclonal Antibody diluted at 1：500

Immunohistochemical analysis of paraffin-embedded Human Right kidney. 1, Antibody was diluted at 1:100（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.