产品名称
14-3-3-pan (Acetyl Lys51/49) Rabbit Polyclonal Antibody
别名
YWHAB; 14-3-3 protein beta/alpha; Protein 1054; Protein kinase C inhibitor protein 1; KCIP-1; YWHAG; 14-3-3 protein gamma; Protein kinase C inhibitor protein 1; KCIP-1; YWHAQ; 14-3-3 protein theta; 14-3-3 protein T-cell; 14-3-3 protein tau; Protein HS1; YWHAZ; 14-3-3 protein zeta/delta; Protein kinase C inhibitor protein 1; KCIP-1; SFN; HME1; 14-3-3 protein sigma; Epithelial cell marker protein 1; Stratifin
基因名称
YWHAB/YWHAG/YWHAQ/YWHAZ/SFN
蛋白名称
14-3-3 protein beta/alpha/14-3-3 protein gamma/14-3-3 protein theta/14-3-3 protein zeta/delta/14-3-3 protein sigma
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=7529
Human Swissprot No.
P31946
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P31946/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=54401
Mouse Swissprot No.
Q9CQV8
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q9CQV8
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=56011
Rat Swissprot Link
http://www.uniprot.org/uniprot/P35213
免疫原
Synthesized acetyl-peptide derived from human 14-3-3-pan around the acetylation site of K51.
特异性
Acetyl-14-3-3-pan (K51/49) Polyclonal Antibody detects endogenous levels of 14-3-3-pan around the acetylation site of K51 protein.
稀释度
WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008],
组织表达
Brain,Colon carcinoma,Kerat
细胞定位
Cytoplasm . Melanosome . Identified by mass spectrometry in melanosome fractions from stage I to stage IV.; Vacuole membrane . (Microbial infection) Upon infection with Chlamydia trachomatis, this protein is associated with the pathogen-containing vacuole membrane where it colocalizes with IncG. .
信号通路
Cell_Cycle_G1S;Cell_Cycle_G2M_DNA;Oocyte meiosis;Neurotrophin;
功能
function:Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis.,PTM:Isoform Short contains a N-acetylmethionine at position 1.,PTM:The alpha, brain-specific form differs from the beta form in being phosphorylated.,similarity:Belongs to the 14-3-3 family.,subcellular location:Identified by mass spectrometry in melanosome fractions from stage I to stage IV.,subunit:Homodimer. Interacts with SSH1 and TORC2/CRTC2. Interacts with ABL1; the interaction results in cytoplasmic location of ABL1 and inhition of cABL-mediated apoptosis. Interacts with ROR2 (dimer); the interaction results in phosphorylation of YWHAB on tyrosine residues.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
