产品名称
Septin 7 Rabbit Polyclonal Antibody
别名
SEPT7; CDC10; Septin-7; CDC10 protein homolog
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=989
Human Swissprot No.
Q16181
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q16181/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=235072
Mouse Swissprot No.
O55131
Mouse Swissprot Link
http://www.uniprot.org/uniprot/O55131
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=64551
Rat Swissprot Link
http://www.uniprot.org/uniprot/Q9WVC0
免疫原
The antiserum was produced against synthesized peptide derived from human SEPT7. AA range:350-399
特异性
Septin 7 Polyclonal Antibody detects endogenous levels of Septin 7 protein.
稀释度
WB 1:500 - 1:2000.IHC-p:1:50-300 ELISA: 1:20000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
septin 7(SEPT7) Homo sapiens This gene encodes a protein that is highly similar to the CDC10 protein of Saccharomyces cerevisiae. The protein also shares similarity with Diff 6 of Drosophila and with H5 of mouse. Each of these similar proteins, including the yeast CDC10, contains a GTP-binding motif. The yeast CDC10 protein is a structural component of the 10 nm filament which lies inside the cytoplasmic membrane and is essential for cytokinesis. This human protein functions in gliomagenesis and in the suppression of glioma cell growth, and it is required for the association of centromere-associated protein E with the kinetochore. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been identified on chromosomes 5, 7, 9, 10, 11, 14, 17 and 19. [provided by RefSeq, Jul 2011],
细胞定位
Cytoplasm . Chromosome, centromere, kinetochore . Cytoplasm, cytoskeleton, spindle . Cleavage furrow . Midbody . Cytoplasm, cytoskeleton, cilium axoneme . Cell projection, cilium, flagellum . Distributed throughout the cytoplasm in prometaphase cells. Associated with the spindle during metaphase. Associated with the central spindle and at the cleavage furrow in anaphase cells. Detected at the midbody in telophase. Associated with actin stress fibers (By similarity). Found in the sperm annulus (PubMed:25588830). .
功能
function:Required for normal progress through mitosis. Involved in cytokinesis. Required for normal association of CENPE with the kinetochore.,similarity:Belongs to the septin family.,subcellular location:Distributed throughout the cytoplasm in prometaphase cells. Associated with the spindle during metaphase. Associated with the central spindle and at the cleavage furrow in anaphase cells. Detected at the midbody in telophase.,subunit:Heterohexamer composed of two heterotrimers containing one copy each of SEPT2, SEPT6 and SEPT7. The asymmetrical heterotrimers associate head-to-head to form a hexameric unit that assembles into filaments. Interacts directly with CENPE and links CENPE to septin filaments composed of SEPT2, SEPT6 and SEPT7.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
