BD-PT4225-SDF-1 Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

SDF-1 Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PT4225

产品名称

SDF-1 Rabbit Polyclonal Antibody

别名

CXCL12; SDF1; SDF1A; SDF1B; Stromal cell-derived factor 1; SDF-1; hSDF-1; C-X-C motif chemokine 12; Intercrine reduced in hepatomas; IRH; hIRH; Pre-B cell growth-stimulating factor; PBSF

类别

常规抗体

基因名称

CXCL12

蛋白名称

Stromal cell-derived factor 1

推荐应用

反应种属

Human,Mouse,Rat

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

6387

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=6387

Human Swissprot No.

P48061

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P48061/entry

Mouse Gene ID

20315

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=20315

Mouse Swissprot No.

P40224

Mouse Swissprot Link

http://www.uniprot.org/uniprot/P40224

免疫原

The antiserum was produced against synthesized peptide derived from human SDF-1. AA range:44-93

特异性

SDF-1 Polyclonal Antibody detects endogenous levels of SDF-1 protein.

稀释度

IF 1:50-200 WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000. Not yet tested in other applications.

预测分子量

10-20kD,

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

C-X-C motif chemokine ligand 12(CXCL12) Homo sapiens This antimicrobial gene encodes a stromal cell-derived alpha chemokine member of the intercrine family. The encoded protein functions as the ligand for the G-protein coupled receptor, chemokine (C-X-C motif) receptor 4, and plays a role in many diverse cellular functions, including embryogenesis, immune surveillance, inflammation response, tissue homeostasis, and tumor growth and metastasis. Mutations in this gene are associated with resistance to human immunodeficiency virus type 1 infections. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014],

组织表达

Isoform Alpha and isoform Beta are ubiquitously expressed, with highest levels detected in liver, pancreas and spleen. Isoform Gamma is mainly expressed in heart, with weak expression detected in several other tissues. Isoform Delta, isoform Epsilon and isoform Theta have highest expression levels in pancreas, with lower levels detected in heart, kidney, liver and spleen.

细胞定位

Secreted.

信号通路

Cytokine-cytokine receptor interaction;Chemokine;Axon guidance;Leukocyte transendothelial migration;Intestinal immune network for IgA production;

功能

function:Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites.,online information:SDF-1 entry,PTM:Processed forms SDF-1-beta(3-72) and SDF-1-alpha(3-67) are produced after secretion by proteolytic cleavage of isoforms Beta and Alpha, respectively. The N-terminal processing is probably achieved by DPP4. Isoform Alpha is first cleaved at the C-terminus to yield a SDF-1-alpha(1-67) intermediate before being processsed at the N-terminus. The C-terminal processing of isoform Alpha is reduced by binding to heparin and, probably, cell surface proteoglycans.,similarity:Belongs to the intercrine alpha (chemokine CxC) family.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunohistochemistry analysis of SDF-1 antibody in paraffin-embedded human brain tissue.

Western blot analysis of lysate from HepG2 cells, using SDF-1 antibody.

Immunofluorescence analysis of human-lung tissue. 1,SDF-1 Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of rat-spleen tissue. 1,SDF-1 Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1,SDF-1 Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,SDF-1 Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1,SDF-1 Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Western Blot analysis of various cells using SDF-1 Polyclonal Antibody diluted at 1：2000

Western Blot analysis of HuvEc cells using SDF-1 Polyclonal Antibody diluted at 1：2000