产品名称
SAP 14 Rabbit Polyclonal Antibody
别名
SF3B14; CGI-110; HSPC175; HT006; Pre-mRNA branch site protein p14; SF3b 14 kDa subunit
蛋白名称
Pre-mRNA branch site protein p14
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=51639
Human Swissprot No.
Q9Y3B4
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q9Y3B4/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=66055
Mouse Swissprot No.
P59708
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P59708
免疫原
The antiserum was produced against synthesized peptide derived from human SF3B14. AA range:76-125
特异性
SAP 14 Polyclonal Antibody detects endogenous levels of SAP 14 protein.
稀释度
WB 1:500 - 1:2000. ELISA: 1:40000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a 14 kDa protein subunit of the splicing factor 3b complex. Splicing factor 3b associates with both the U2 and U11/U12 small nuclear ribonucleoprotein complexes (U2 snRNP) of spliceosomes. This 14 kDa protein interacts directly with subunit 1 of the splicing factor 3b complex. This 14 kDa protein also interacts directly with the adenosine that carries out the first transesterification step of splicing at the pre-mRNA branch site. [provided by RefSeq, Jul 2008],
组织表达
Hypothalamus,Testis,Umbilical cord blood,Uterus,
功能
function:Necessary for the splicing of pre-mRNA. Directly contacts the pre-mRNA branch site adenosine for the first catalytic step of splicing. Enters the spliceosome and associates with the pre-mRNA branch site as part of the 17S U2 or, in the case of the minor spliceosome, as part of the 18S U11/U12 snRNP complex, and thus may facilitate the interaction of these snRNP with the branch sites of U2 and U12 respectively.,similarity:Contains 1 RRM (RNA recognition motif) domain.,subunit:Interacts with SF3B1/SF3b155 in the region of amino acids 255-424. Interacts to a lesser extent with SF3b130.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
