产品名称
SAE1 Rabbit Polyclonal Antibody
别名
SAE1; AOS1; SUA1; UBLE1A; SUMO-activating enzyme subunit 1; Ubiquitin-like 1-activating enzyme E1A
蛋白名称
SUMO-activating enzyme subunit 1
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=10055
Human Swissprot No.
Q9UBE0
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q9UBE0/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=56459
Mouse Swissprot No.
Q9R1T2
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q9R1T2
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=308384
Rat Swissprot Link
http://www.uniprot.org/uniprot/Q6AXQ0
免疫原
The antiserum was produced against synthesized peptide derived from human AOS1. AA range:221-270
特异性
SAE1 Polyclonal Antibody detects endogenous levels of SAE1 protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
SUMO1 activating enzyme subunit 1(SAE1) Homo sapiens Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 and UBA2 (MIM 613295) form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).[supplied by OMIM, Mar 2010],
组织表达
Expression level increases during S phase and drops in G2 phase (at protein level).
信号通路
Ubiquitin mediated proteolysis;
功能
function:The dimeric enzyme acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins and formation of a thioester with a conserved cysteine residue on SAE2.,pathway:Protein modification; protein sumoylation.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the ubiquitin-activating E1 family.,subunit:Heterodimer of SAE1 and SAE2. The complex binds SUMO proteins via SAE2.,tissue specificity:Expression level increases during S phase and drops in G2 phase (at protein level).,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
