产品名称
S2P Rabbit Polyclonal Antibody
别名
MBTPS2; S2P; Membrane-bound transcription factor site-2 protease; Endopeptidase S2P; Sterol regulatory element-binding proteins intramembrane protease; SREBPs intramembrane protease
蛋白名称
Membrane-bound transcription factor site-2 protease
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=51360
Human Swissprot No.
O43462
Human Swissprot Link
http://www.uniprot.org/uniprotkb/O43462/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=270669
Mouse Swissprot No.
Q8CHX6
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q8CHX6
免疫原
The antiserum was produced against synthesized peptide derived from human MBTPS2. AA range:301-350
特异性
S2P Polyclonal Antibody detects endogenous levels of S2P protein.
稀释度
WB 1:500-2000 IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a intramembrane zinc metalloprotease, which is essential in development. This protease functions in the signal protein activation involved in sterol control of transcription and the ER stress response. Mutations in this gene have been associated with ichthyosis follicularis with atrichia and photophobia (IFAP syndrome); IFAP syndrome has been quantitatively linked to a reduction in cholesterol homeostasis and ER stress response.[provided by RefSeq, Aug 2009],
组织表达
Expressed in heart, brain, placenta, lung, liver, muscle, kidney and pancreas.
细胞定位
Membrane ; Multi-pass membrane protein . Cytoplasm .
功能
catalytic activity:Cleaves several transcription factors that are type-2 transmembrane proteins within membrane-spanning domains. Known substrates include sterol regulatory element-binding protein (SREBP) -1, SREBP-2 and forms of the transcriptional activator ATF6. SREBP-2 is cleaved at the site 477-DRSRILL-|-CVLTFLCLSFNPLTSLLQWGGA-505. The residues Asn-Pro, 11 residues distal to the site of cleavage in the membrane-spanning domain, are important for cleavage by S2P endopeptidase. Replacement of either of these residues does not prevent cleavage, but there is no cleavage if both of these residues are replaced.,cofactor:Binds 1 zinc ion per subunit.,function:Intramembrane proteolysis of sterol-regulatory element-binding proteins (SREBPs) within the first transmembrane segment thereby releasing the N-terminal segment with a portion of the transmembrane segment attached. Site-2 cleavage comes after site-1 cleavage which takes place in the lumenal loop.,similarity:Belongs to the peptidase M50A family.,tissue specificity:Expressed in heart, brain, placenta, lung, liver, muscle, kidney and pancreas.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
