BD-PT4199-S-100A16 Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

S-100A16 Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PT4199

产品名称

S-100A16 Rabbit Polyclonal Antibody

别名

S100A16; S100F; AAG13; Protein S100-A16; Aging-associated gene 13 protein; Protein S100-F; S100 calcium-binding protein A16

类别

常规抗体

基因名称

S100A16

蛋白名称

Protein S100-A16

推荐应用

反应种属

Human,Rat

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

140576

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=140576

Human Swissprot No.

Q96FQ6

Human Swissprot Link

http://www.uniprot.org/uniprotkb/Q96FQ6/entry

免疫原

The antiserum was produced against synthesized peptide derived from human S100A16. AA range:19-68

特异性

S-100A16 Polyclonal Antibody detects endogenous levels of S-100A16 protein.

稀释度

WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200

预测分子量

20kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

similarity:Belongs to the S-100 family.,similarity:Contains 2 EF-hand domains.,

组织表达

Ubiquitous (PubMed:14684152). Highly expressed in esophagus, adipose tissues and colon. Expressed at lower level in lung, brain, pancreas and skeletal muscle. Expression is up-regulated in tumors of bladder, lung, thyroid gland, pancreas and ovary (PubMed:14684152). Expressed in astrocytes (PubMed:17030513).

细胞定位

Nucleus, nucleolus . Cytoplasm . Primarily nucleolar. A high intracellular calcium level induces nucleolar exit and nucleocytoplasmic transport, whereas a low intracellular calcium level leads to nuclear translocation and accumulation within specific region of nucleoli (PubMed:17030513).

功能

similarity:Belongs to the S-100 family.,similarity:Contains 2 EF-hand domains.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Western blot analysis of lysates from NIH/3T3 and RAW264.7 cells, using S100A16 Antibody. The lane on the right is blocked with the synthesized peptide.

Western blot analysis of the lysates from RAW264.7cells using S100A16 antibody.

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100（4°,overnight）. High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl （right） obtaned from antibody was pre-absorbed by immunogen peptide.

Immunohistochemical analysis of paraffin-embedded Human cervix cancer. Antibody was diluted at 1:100（4°,overnight）. High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl （right） obtaned from antibody was pre-absorbed by immunogen peptide.