产品名称
S-100 α Rabbit Polyclonal Antibody
别名
S100A1; S100A; Protein S100-A1; S-100 protein alpha chain; S-100 protein subunit alpha; S100 calcium-binding protein A1
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=6271
Human Swissprot No.
P23297
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P23297/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=20193
Mouse Swissprot No.
P56565
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P56565
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=295214
Rat Swissprot Link
http://www.uniprot.org/uniprot/P35467
免疫原
The antiserum was produced against synthesized peptide derived from human S100 A1. AA range:10-59
特异性
S-100 α Polyclonal Antibody detects endogenous levels of S-100 α protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
S100 calcium binding protein A1(S100A1) Homo sapiens The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in stimulation of Ca2+-induced Ca2+ release, inhibition of microtubule assembly, and inhibition of protein kinase C-mediated phosphorylation. Reduced expression of this protein has been implicated in cardiomyopathies. [provided by RefSeq, Jul 2008],
组织表达
Highly prevalent in heart (PubMed:12804600, PubMed:1384693). Also found in lesser quantities in skeletal muscle and brain (PubMed:1384693).
细胞定位
Cytoplasm . Sarcoplasmic reticulum . Mitochondrion .
功能
function:Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.,similarity:Belongs to the S-100 family.,similarity:Contains 2 EF-hand domains.,subunit:Dimer of either two alpha chains, or two beta chains, or one alpha and one beta chain.,tissue specificity:Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
