产品名称
RUFY1 Rabbit Polyclonal Antibody
别名
RUFY1; RABIP4; ZFYVE12; RUN and FYVE domain-containing protein 1; FYVE-finger protein EIP1; La-binding protein 1; Rab4-interacting protein; Zinc finger FYVE domain-containing protein 12
蛋白名称
RUN and FYVE domain-containing protein 1
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=80230
Human Swissprot No.
Q96T51
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q96T51/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=216724
Mouse Swissprot No.
Q8BIJ7
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q8BIJ7
免疫原
The antiserum was produced against synthesized peptide derived from human RUFY1. AA range:139-188
特异性
RUFY1 Polyclonal Antibody detects endogenous levels of RUFY1 protein.
稀释度
WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
RUN and FYVE domain containing 1(RUFY1) Homo sapiens This gene encodes a protein that contains a RUN domain and a FYVE-type zinc finger domain. The encoded protein binds to phosphatidylinositol-3-phosphate (PI3P) and plays a role in early endosomal trafficking, tethering and fusion through interactions with small GTPases including Rab4, Rab5 and Rab14. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011],
组织表达
Broadly expressed, with highest levels in lung, testis, kidney and brain.
细胞定位
Cytoplasm. Early endosome membrane; Peripheral membrane protein.
功能
domain:The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate.,function:Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in early endosomal trafficking.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,PTM:Phosphorylation on Tyr-389 and/or Tyr-400 is required for interaction with BMX and endosomal targeting.,similarity:Contains 1 FYVE-type zinc finger.,similarity:Contains 1 RUN domain.,subunit:Interacts with BMX. May interact with SSB. Interacts with RAB4 and RAB5 that have been activated by GTP-binding.,tissue specificity:Broadly expressed, with highest levels in lung, testis, kidney and brain.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
