BD-PK0060

HMG-1 (Acetyl Lys12) Rabbit Polyclonal Antibody

HMGB1; HMG1; High mobility group protein B1; High mobility group protein 1; HMG-1

常规抗体

HMGB1

High mobility group protein B1

WB

Human,Mouse,Rat

1 mg/ml

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

3146

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=3146

P09429

http://www.uniprot.org/uniprotkb/P09429/entry

100862258

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=100862258

P63158

http://www.uniprot.org/uniprot/P63158

25459

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=25459

P63159

http://www.uniprot.org/uniprot/P63159

Synthesized acetyl-peptide derived from the N-terminal region of human HMG-1 around the acetylation site of K12.

Acetyl-HMG-1 (K12) Polyclonal Antibody detects endogenous levels of HMG-1 protein only when acetylation at K12.

WB 1:500 - 1:2000. IHC-p: 1:100-300 ELISA: 1:10000.. IF 1:50-200

about 30kd

-20°C/1 year

Polyclonal, Rabbit,IgG

This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2015],

Ubiquitous. Expressed in platelets (PubMed:11154118).

Nucleus . Chromosome . Cytoplasm . Secreted . Cell membrane ; Peripheral membrane protein ; Extracellular side . Endosome . Endoplasmic reticulum-Golgi intermediate compartment . In basal state predominantly nuclear. Shuttles between the cytoplasm and the nucleus (PubMed:12231511, PubMed:17114460). Translocates from the nucleus to the cytoplasm upon autophagy stimulation (PubMed:20819940). Release from macrophages in the extracellular milieu requires the activation of NLRC4 or NLRP3 inflammasomes (By similarity). Passively released to the extracellular milieu from necrotic cells by diffusion, involving the fully reduced HGMB1 which subsequently gets oxidized (PubMed:19811284). Also released from apoptotic cells (PubMed:16855214, PubMed:18631454). Active secretion from a variety of immune and non-immune cells such as macrophages, monocytes, neutrophils, dendritic cells and natural killer cells in response to various stimuli such as LPS and cytokines involves a nonconventional secretory process via secretory lysosomes (PubMed:12231511, PubMed:14532127, PubMed:15944249). Secreted by plasma cells in response to LPS (By similarity). Found on the surface of activated platelets (PubMed:11154118). An increased chromatin association is observed when associated with the adenovirus protein pVII (PubMed:27362237). .

Base excision repair;

function:Binds preferentially single-stranded DNA and unwinds double stranded DNA.,similarity:Belongs to the HMGB family.,similarity:Contains 2 HMG box DNA-binding domains.,

现货

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.