产品名称
RTCD1 Rabbit Polyclonal Antibody
别名
RTCA; RPC; RPC1; RTC1; RTCD1; RNA 3'-terminal phosphate cyclase; RNA cyclase; RNA-3'-phosphate cyclase; RNA terminal phosphate cyclase domain-containing protein 1; RTC domain-containing protein 1
蛋白名称
RNA 3'-terminal phosphate cyclase
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=8634
Human Swissprot No.
O00442
Human Swissprot Link
http://www.uniprot.org/uniprotkb/O00442/entry
Mouse Swissprot No.
Q9D7H3
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q9D7H3
免疫原
The antiserum was produced against synthesized peptide derived from human RTCD1. AA range:317-366
特异性
RTCD1 Polyclonal Antibody detects endogenous levels of RTCD1 protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
RNA 3'-terminal phosphate cyclase(RTCA) Homo sapiens This gene encodes a member of the RNA 3'-phosphate cyclase family. The encoded protein plays a role in RNA metabolism by catalyzing the ATP-dependent conversion of the 3'-phosphate of RNA substrates to a 2',3'-cyclic phosphodiester. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2012],
细胞定位
Nucleus, nucleoplasm .
功能
catalytic activity:ATP + RNA 3'-terminal-phosphate = AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate.,function:Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) the enzyme acts on RNA-N3'P to produce RNA-N3'PP5'A; (C) a non catalytic nucleophilic attack by the adjacent 2'hydroxyl on the phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing.,similarity:Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.,subunit:Monomer.,tissue specificity:Ubiquitous.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
