产品名称
RPA135 Rabbit Polyclonal Antibody
别名
POLR1B; DNA-directed RNA polymerase I subunit RPA2; RNA polymerase I subunit 2; DNA-directed RNA polymerase I 135 kDa polypeptide; RPA135
蛋白名称
DNA-directed RNA polymerase I subunit RPA2
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=84172
Human Swissprot No.
Q9H9Y6
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q9H9Y6/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=20017
Mouse Swissprot No.
P70700
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P70700
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=83582
Rat Swissprot Link
http://www.uniprot.org/uniprot/O54888
免疫原
Synthesized peptide derived from the Internal region of human RPA135.
特异性
RPA135 Polyclonal Antibody detects endogenous levels of RPA135 protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
Eukaryotic RNA polymerase I (pol I) is responsible for the transcription of ribosomal RNA (rRNA) genes and production of rRNA, the primary component of ribosomes. Pol I is a multisubunit enzyme composed of 6 to 14 polypeptides, depending on the species. Most of the mass of the pol I complex derives from the 2 largest subunits, Rpa1 and Rpa2 in yeast. POLR1B is homologous to Rpa2 (Seither and Grummt, 1996 [PubMed 8921381]).[supplied by OMIM, Mar 2008],
组织表达
Eye,Pancreas,Skin,Testis,
细胞定位
Nucleus, nucleolus . Chromosome .
信号通路
Purine metabolism;Pyrimidine metabolism;RNA polymerase;
功能
catalytic activity:Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).,function:DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol I is composed of mobile elements and RPA2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft.,similarity:Belongs to the RNA polymerase beta chain family.,subunit:Component of the RNA polymerase I (Pol I) complex consisting of at least 13 subunits.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
