产品名称
Rock-2 Rabbit Polyclonal Antibody
别名
ROCK2; KIAA0619; Rho-associated protein kinase 2; Rho kinase 2; Rho-associated; coiled-coil-containing protein kinase 2; Rho-associated, coiled-coil-containing protein kinase II; ROCK-II; p164 ROCK-2
蛋白名称
Rho-associated protein kinase 2
反应种属
Human,Mouse,Rat,Monkey
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=9475
Human Swissprot No.
O75116
Human Swissprot Link
http://www.uniprot.org/uniprotkb/O75116/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=19878
Mouse Swissprot No.
P70336
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P70336
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=25537
Rat Swissprot Link
http://www.uniprot.org/uniprot/Q62868
免疫原
The antiserum was produced against synthesized peptide derived from human ROCK2. AA range:691-740
特异性
Rock-2 Polyclonal Antibody detects endogenous levels of Rock-2 protein.
稀释度
IF 1:50-200 WB 1:500 - 1:2000. IHC 1:100 - 1:300. Immunocytochemistry: 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. [provided by RefSeq, Jul 2008],
组织表达
Expressed in the brain (at protein level).
细胞定位
Cytoplasm. Cell membrane ; Peripheral membrane protein . Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasmic, and associated with actin microfilaments and the plasma membrane. .
信号通路
Chemokine;Vascular smooth muscle contraction;WNT;WNT-T CELLTGF-beta;Axon guidance;Focal adhesion;Leukocyte transendothelial migration;Regulates Actin and Cytoskeleton;Pathogenic Escherichia coli infection;
功能
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,enzyme regulation:Activated by RHOA binding.,function:Protein kinase that phosphorylates a large number of important signaling proteins, and thereby regulates the assembly of the actin cytoskeleton. Promotes formation of stress fibers and of focal adhesion complexes. Plays a role in smooth muscle contraction.,miscellaneous:Inhibited by Y-27632.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.,similarity:Contains 1 AGC-kinase C-terminal domain.,similarity:Contains 1 PH domain.,similarity:Contains 1 phorbol-ester/DAG-type zinc finger.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 REM (Hr1) repeat.,subcellular location:Cytoplasmic, and associated with actin microfilaments and the plasma membrane.,subunit:Homodimer. Binds RHOA that has been activated by GTP binding. Binds IRS1, RHOB and RHOC.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
