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Ribosomal Protein S4Y1 Rabbit Polyclonal Antibody

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产品基本信息

产品货号
BD-PT4136
产品名称
Ribosomal Protein S4Y1 Rabbit Polyclonal Antibody
别名
RPS4Y1; RPS4Y; 40S ribosomal protein S4; Y isoform 1
类别
常规抗体
基因名称
RPS4Y1
蛋白名称
40S ribosomal protein S4 Y isoform 1
推荐应用
WB
反应种属
Human,Rat,Mouse
浓度
1 mg/ml
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene ID
6192
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=6192
Human Swissprot No.
P22090
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P22090/entry
免疫原
The antiserum was produced against synthesized peptide derived from human RPS4Y1. AA range:214-263
特异性
Ribosomal Protein S4Y1 Polyclonal Antibody detects endogenous levels of Ribosomal Protein S4Y1 protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
预测分子量
29kD
运输及保存条件
-20°C/1 year
宿主
Polyclonal, Rabbit,IgG
背景介绍
Cytoplasmic ribosomes, organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes ribosomal protein S4, a component of the 40S subunit. Ribosomal protein S4 is the only ribosomal protein known to be encoded by more than one gene, namely this gene and ribosomal protein S4, X-linked (RPS4X). The 2 isoforms encoded by these genes are not identical, but are functionally equivalent. Ribosomal protein S4 belongs to the S4E family of ribosomal proteins. It has been suggested that haploinsufficiency of the ribosomal protein S4 genes plays a role in Turner syndrome; however, this hypothesis is controversial. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed throug
组织表达
Coronary artery,Fetal liver,PCR rescued clones,Placenta,
细胞定位
nucleus,nucleoplasm,cytosol,ribosome,polysome,membrane,cytosolic small ribosomal subunit,
信号通路
Ribosome;
功能
miscellaneous:This isoform is encoded on the Y chromosome.,similarity:Belongs to the ribosomal protein S4e family.,similarity:Contains 1 S4 RNA-binding domain.,
期货
现货
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunofluorescence analysis of HUVEC cells, using RPS4Y1 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from HUVEC, HeLa, and COLO cells, using RPS4Y1 Antibody. The lane on the right is blocked with the synthesized peptide.

Western Blot analysis of various cells using Ribosomal Protein S4Y1 Polyclonal Antibody diluted at 1:1000

Western blot analysis of 293T mouse-spleen lysis using Ribosomal Protein S4Y1 antibody. Antibody was diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4°,overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

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