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Ribosomal Protein S20 Rabbit Polyclonal Antibody

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产品基本信息

产品货号
BD-PT4129
产品名称
Ribosomal Protein S20 Rabbit Polyclonal Antibody
别名
RPS20; 40S ribosomal protein S20
类别
常规抗体
基因名称
RPS20
蛋白名称
40S ribosomal protein S20
推荐应用
WB
反应种属
Human,Mouse,Rat
浓度
1 mg/ml
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene ID
6224
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=6224
Human Swissprot No.
P60866
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P60866/entry
Mouse Gene ID
67427
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=67427
Mouse Swissprot No.
P60867
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P60867
Rat Gene ID
100360000
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=100359951
Rat Swissprot No.
P60868
Rat Swissprot Link
http://www.uniprot.org/uniprot/P60868
免疫原
The antiserum was produced against synthesized peptide derived from human RPS20. AA range:31-80
特异性
Ribosomal Protein S20 Polyclonal Antibody detects endogenous levels of Ribosomal Protein S20 protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.
预测分子量
13kD
运输及保存条件
-20°C/1 year
宿主
Polyclonal, Rabbit,IgG
背景介绍
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S10P family of ribosomal proteins. It is located in the cytoplasm. This gene is co-transcribed with the small nucleolar RNA gene U54, which is located in its second intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Two transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Apr 2009],
组织表达
Colon adenocarcinoma,Ovary,Placenta,Synovial membrane tissue,Testis,
细胞定位
Cytoplasm .
信号通路
Ribosome;
功能
similarity:Belongs to the ribosomal protein S10P family.,
期货
现货
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunofluorescence analysis of A549 cells, using RPS20 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from HUVEC and MCF-7 cells, using RPS20 Antibody. The lane on the right is blocked with the synthesized peptide.

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4°,overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

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