BD-PT4122-Ribosomal Protein S12 Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

Ribosomal Protein S12 Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PT4122

产品名称

Ribosomal Protein S12 Rabbit Polyclonal Antibody

别名

RPS12; 40S ribosomal protein S12

类别

常规抗体

基因名称

RPS12

蛋白名称

40S ribosomal protein S12

推荐应用

反应种属

Human,Mouse,Rat

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

6206

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=6206

Human Swissprot No.

P25398

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P25398/entry

Mouse Swissprot No.

P63323

Mouse Swissprot Link

http://www.uniprot.org/uniprot/P63323

Rat Swissprot No.

P63324

Rat Swissprot Link

http://www.uniprot.org/uniprot/P63324

免疫原

The antiserum was produced against synthesized peptide derived from human RPS12. AA range:83-132

特异性

Ribosomal Protein S12 Polyclonal Antibody detects endogenous levels of Ribosomal Protein S12 protein.

稀释度

WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.

预测分子量

15kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S12E family of ribosomal proteins. It is located in the cytoplasm. Increased expression of this gene in colorectal cancers compared to matched normal colonic mucosa has been observed. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008],

组织表达

Cervix carcinoma,Embryonic stem cell,Hepatoma,Mammary carcinoma,Placenta,Skin,Testi

细胞定位

Cytoplasm.

信号通路

Ribosome;

功能

similarity:Belongs to the ribosomal protein S12e family.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunofluorescence analysis of A549 cells, using RPS12 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from COLO cells, using RPS12 Antibody. The lane on the right is blocked with the synthesized peptide.

Western blot analysis of the lysates from HUVECcells using RPS12 antibody.

Western Blot analysis of various cells using Ribosomal Protein S12 Polyclonal Antibody

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100（4°,overnight）. High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl （right） obtaned from antibody was pre-absorbed by immunogen peptide.