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Ribosomal Protein L34 Rabbit Polyclonal Antibody

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产品基本信息

产品货号
BD-PT4108
产品名称
Ribosomal Protein L34 Rabbit Polyclonal Antibody
别名
RPL34; 60S ribosomal protein L34
类别
常规抗体
基因名称
RPL34
蛋白名称
60S ribosomal protein L34
推荐应用
WB
反应种属
Human,Mouse,Rat
浓度
1 mg/ml
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene ID
6164
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=6164
Human Swissprot No.
P49207
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P49207/entry
Mouse Gene ID
100044000
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=100043876
Mouse Swissprot No.
Q9D1R9
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q9D1R9
Rat Swissprot No.
P11250
Rat Swissprot Link
http://www.uniprot.org/uniprot/P11250
免疫原
The antiserum was produced against synthesized peptide derived from human RPL34. AA range:41-90
特异性
Ribosomal Protein L34 Polyclonal Antibody detects endogenous levels of Ribosomal Protein L34 protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
预测分子量
13kD
运输及保存条件
-20°C/1 year
宿主
Polyclonal, Rabbit,IgG
背景介绍
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L34E family of ribosomal proteins. It is located in the cytoplasm. This gene originally was thought to be located at 17q21, but it has been mapped to 4q. Overexpression of this gene has been observed in some cancer cells. Alternative splicing results in multiple transcript variants, all encoding the same isoform. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Feb 2016],
组织表达
Bone,Epithelium,Eye,Ovary,
细胞定位
Cytoplasm, cytosol . Cytoplasm . Endoplasmic reticulum . Detected on cytosolic polysomes (PubMed:25957688). Detected in ribosomes that are associated with the rough endoplasmic reticulum (By similarity). .
信号通路
Ribosome;
功能
similarity:Belongs to the ribosomal protein L34e family.,
期货
现货
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunofluorescence analysis of HUVEC cells, using RPL34 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from HepG2, HeLa, and HUVEC cells, using RPL34 Antibody. The lane on the right is blocked with the synthesized peptide.

Western Blot analysis of various cells using Ribosomal Protein L34 Polyclonal Antibody diluted at 1:500

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4°,overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

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