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Ribosomal Protein L28 Rabbit Polyclonal Antibody

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产品基本信息

产品货号
BD-PT4104
产品名称
Ribosomal Protein L28 Rabbit Polyclonal Antibody
别名
RPL28; 60S ribosomal protein L28
类别
常规抗体
基因名称
RPL28
蛋白名称
60S ribosomal protein L28
推荐应用
WB
反应种属
Human,Mouse,Rat
浓度
1 mg/ml
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene ID
6158
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=6158
Human Swissprot No.
P46779
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P46779/entry
Mouse Gene ID
19943
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=19943
Mouse Swissprot No.
P41105
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P41105
Rat Swissprot No.
P17702
Rat Swissprot Link
http://www.uniprot.org/uniprot/P17702
免疫原
The antiserum was produced against synthesized peptide derived from human RPL28. AA range:41-90
特异性
Ribosomal Protein L28 Polyclonal Antibody detects endogenous levels of Ribosomal Protein L28 protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
预测分子量
20kD
运输及保存条件
-20°C/1 year
宿主
Polyclonal, Rabbit,IgG
背景介绍
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L28E family of ribosomal proteins. It is located in the cytoplasm. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Oct 2008],
组织表达
B-cell,Cerebellum,Colon,Hepatoma,Kidney,Ovarian carcinoma,Spleen,Uterus,
细胞定位
cytoplasm,cytosol,ribosome,membrane,cytosolic large ribosomal subunit,dendrite,cytoplasmic ribonucleoprotein granule,cell body,extracellular exosome,
信号通路
Ribosome;
功能
similarity:Belongs to the ribosomal protein L28e family.,
期货
现货
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Western blot analysis of lysates from A549, 293, and COLO cells, using RPL28 Antibody. The lane on the right is blocked with the synthesized peptide.

Western blot analysis of the lysates from HeLa cells using RPL28 antibody.

Western Blot analysis of various cells using Ribosomal Protein L28 Polyclonal Antibody diluted at 1:2000

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