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Ribosomal Protein L27A Rabbit Polyclonal Antibody

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产品基本信息

产品货号
BD-PT4103
产品名称
Ribosomal Protein L27A Rabbit Polyclonal Antibody
别名
RPL27A; 60S ribosomal protein L27a
类别
常规抗体
基因名称
RPL27A
蛋白名称
60S ribosomal protein L27a
推荐应用
WB
反应种属
Human,Mouse,Rat
浓度
1 mg/ml
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene ID
6157
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=6157
Human Swissprot No.
P46776
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P46776/entry
Mouse Gene ID
26451
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=26451
Mouse Swissprot No.
P14115
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P14115
Rat Gene ID
293418
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=293418
Rat Swissprot No.
P18445
Rat Swissprot Link
http://www.uniprot.org/uniprot/P18445
免疫原
The antiserum was produced against synthesized peptide derived from human RPL27A. AA range:99-148
特异性
Ribosomal Protein L27A Polyclonal Antibody detects endogenous levels of Ribosomal Protein L27A protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.
预测分子量
20kD
运输及保存条件
-20°C/1 year
宿主
Polyclonal, Rabbit,IgG
背景介绍
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L15P family of ribosomal proteins. It is located in the cytoplasm. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. As is typical for genes encoding ribosomal proteins, multiple processed pseudogenes derived from this gene are dispersed through the genome. [provided by RefSeq, Jul 2008],
组织表达
Brain,Colon,Kidney,Lymph,
细胞定位
cytosol,ribosome,large ribosomal subunit,membrane,cytosolic large ribosomal subunit,
信号通路
Ribosome;
功能
similarity:Belongs to the ribosomal protein L15P family.,
期货
现货
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunofluorescence analysis of A549 cells, using RPL27A Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from COLO cells, using RPL27A Antibody. The lane on the right is blocked with the synthesized peptide.

Western Blot analysis of various cells using Ribosomal Protein L27A Polyclonal Antibody diluted at 1:2000

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4°,overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

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