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Ribosomal Protein L12 Rabbit Polyclonal Antibody

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产品基本信息

产品货号
BD-PT4095
产品名称
Ribosomal Protein L12 Rabbit Polyclonal Antibody
别名
RPL12; 60S ribosomal protein L12
类别
常规抗体
基因名称
RPL12
蛋白名称
60S ribosomal protein L12
推荐应用
WB
反应种属
Human,Mouse,Rat
浓度
1 mg/ml
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene ID
6136
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=6136
Human Swissprot No.
P30050
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P30050/entry
Mouse Gene ID
269261
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=269261
Mouse Swissprot No.
P35979
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P35979
Rat Gene ID
499782
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=499782
Rat Swissprot No.
P23358
Rat Swissprot Link
http://www.uniprot.org/uniprot/P23358
免疫原
The antiserum was produced against synthesized peptide derived from human RPL12. AA range:61-110
特异性
Ribosomal Protein L12 Polyclonal Antibody detects endogenous levels of Ribosomal Protein L12 protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200
预测分子量
18kD
运输及保存条件
-20°C/1 year
宿主
Polyclonal, Rabbit,IgG
背景介绍
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L11P family of ribosomal proteins. It is located in the cytoplasm. The protein binds directly to the 26S rRNA. This gene is co-transcribed with the U65 snoRNA, which is located in its fourth intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008],
组织表达
Brain,Epithelium,Ovary,
细胞定位
nucleolus,cytosol,focal adhesion,membrane,cytosolic large ribosomal subunit,extracellular matrix,extracellular exosome,
信号通路
Ribosome;
功能
function:Binds directly to 26S ribosomal RNA.,similarity:Belongs to the ribosomal protein L11P family.,
期货
现货
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Western blot analysis of lysates from HepG2 and HeLa cells, using RPL12 Antibody. The lane on the right is blocked with the synthesized peptide.

Western Blot analysis of various cells using Ribosomal Protein L12 Polyclonal Antibody diluted at 1:2000

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4°,overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

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