产品名称
RhoGEF p115 Rabbit Polyclonal Antibody
别名
ARHGEF1; Rho guanine nucleotide exchange factor 1; 115 kDa guanine nucleotide exchange factor; p115-RhoGEF; p115RhoGEF; Sub1.5
蛋白名称
Rho guanine nucleotide exchange factor 1
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=9138
Human Swissprot No.
Q92888
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q92888/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=16801
Mouse Swissprot No.
Q61210
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q61210
Rat Swissprot Link
http://www.uniprot.org/uniprot/Q9Z1I6
免疫原
The antiserum was produced against synthesized peptide derived from human ARHGEF1. AA range:162-211
特异性
RhoGEF p115 Polyclonal Antibody detects endogenous levels of RhoGEF p115 protein.
稀释度
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate Rho-dependent signals. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined. [provided by RefSeq, Jul 2008],
组织表达
Ubiquitously expressed.
细胞定位
Cytoplasm . Membrane . Translocated to the membrane by activated GNA13 or LPA stimulation.
信号通路
Regulation of Actin Dynamics; AMPK
功能
domain:The DH domain is involved in interaction with CCPG1.,domain:The RGSL domain, also known as rgRGS domain, is necessary but not sufficient for GAP activity.,function:Seems to play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13) subunits. Acts as GTPase-activating protein (GAP) for GNA12 and GNA13, and as guanine nucleotide exchange factor (GEF) for RhoA GTPase. Activated G alpha 13/GNA13 stimulates the RhoGEF activity through interaction with the RGS-like domain. This GEF activity is inhibited by binding to activated GNA12.,PTM:Phosphorylated by PKCA.,sequence caution:Contaminating sequence. Sequence of unknown origin in the N-terminal part.,similarity:Contains 1 DH (DBL-homology) domain.,similarity:Contains 1 PH domain.,similarity:Contains 1 RGSL (RGS-like) domain.,subcellular location:Translocated to the membrane by activated GNA13 or LPA stimulation.,subunit:Interacts with RHOA, GNA12 and GNA13. Homooligomerizes through the coiled coil region. May interact with CCPG1 (By similarity). Interacts with CTNNAL1.,tissue specificity:Ubiquitously expressed.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
