产品名称
Rho G Rabbit Polyclonal Antibody
别名
RHOG; ARHG; Rho-related GTP-binding protein RhoG
蛋白名称
Rho-related GTP-binding protein RhoG
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=391
Human Swissprot No.
P84095
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P84095/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=56212
Mouse Swissprot No.
P84096
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P84096
免疫原
The antiserum was produced against synthesized peptide derived from human RHOG. AA range:97-146
特异性
Rho G Polyclonal Antibody detects endogenous levels of Rho G protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. The encoded protein facilitates translocation of a functional guanine nucleotide exchange factor (GEF) complex from the cytoplasm to the plasma membrane where ras-related C3 botulinum toxin substrate 1 is activated to promote lamellipodium formation and cell migration. Two related pseudogene have been identified on chromosomes 20 and X. [provided by RefSeq, Aug 2011],
组织表达
Brain,PCR rescued clones,
细胞定位
Cell membrane ; Lipid-anchor ; Cytoplasmic side .
功能
function:Required for the formation of membrane ruffles during macropinocytosis. Required for the formation of cup-like structures during trans-endothelial migration of leukocytes. In case of Salmonella enterica infection, activated by SopB and SGEF, which induces cytoskeleton rearrangements and promotes bacterial entry.,similarity:Belongs to the small GTPase superfamily. Rho family.,subunit:Interacts with SGEF.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
