BD-PT4077-Rho A Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

Rho A Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PT4077

产品名称

Rho A Rabbit Polyclonal Antibody

别名

RHOA; ARH12; ARHA; RHO12; Transforming protein RhoA; Rho cDNA clone 12; h12

类别

常规抗体

基因名称

RHOA

蛋白名称

Transforming protein RhoA

推荐应用

反应种属

Human,Mouse,Rat

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

387

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=387

Human Swissprot No.

P61586

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P61586/entry

Mouse Gene ID

11848

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=11848

Mouse Swissprot No.

Q9QUI0

Mouse Swissprot Link

http://www.uniprot.org/uniprot/Q9QUI0

Rat Gene ID

117273

Rat Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=117273

Rat Swissprot No.

P61589

Rat Swissprot Link

http://www.uniprot.org/uniprot/P61589

免疫原

The antiserum was produced against synthesized peptide derived from human RhoA. AA range:144-193

特异性

Rho A Polyclonal Antibody detects endogenous levels of Rho A protein.

稀释度

IF 1:50-200 WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000. Not yet tested in other applications.

预测分子量

22kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015],

组织表达

Adipose tissue,Brain,Colon,Mammary cancer,Oesophageal carcinoma,Placenta,Re

细胞定位

Cell membrane; Lipid-anchor; Cytoplasmic side. Cytoplasm, cytoskeleton. Cleavage furrow. Cytoplasm, cell cortex . Midbody. Cell projection, lamellipodium . Cell projection, dendrite . Nucleus . Localized to cell-cell contacts in calcium-treated keratinocytes (By similarity). Translocates to the equatorial region before furrow formation in a ECT2-dependent manner. Localizes to the equatorial cell cortex (at the site of the presumptive furrow) in early anaphase in an activated form and in a myosin- and actin-independent manner. .

信号通路

Chemokine;Vascular smooth muscle contraction;WNT;WNT-T CELLTGF-beta;Axon guidance;Focal adhesion;Adherens_Junction;Adherens_Junction;T_Cell_Receptor;Leukocyte transendothelial migration;Neurotrophin;Regulates Actin and Cytoskeleton;Pathogenic Escherichia

功能

cofactor:Magnesium.,domain:The basic-rich region is essential for yopT recognition and cleavage.,function:Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP.,PTM:Cleaved by yopT protease when the cell is infected by some Yersinia pathogens. This removes the lipid attachment, and leads to its displacement from plasma membrane and to subsequent cytoskeleton cleavage.,PTM:Substrate for botulinum ADP-ribosyltransferase.,similarity:Belongs to the small GTPase superfamily. Rho family.,subunit:Interacts with RGNEF (By similarity). Binds PRKCL1, ROCK1 and ROCK2. Interacts with ARHGEF2, ARHGEF3, NET1 and RTKN. Interacts with PLCE1 and AKAP13. Interacts with human respiratory syncytial virus (HRSV) protein F; this interaction facilitates virus-induced syncytium formation.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using RhoA Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from HepG2 cells, using RhoA Antibody. The lane on the right is blocked with the synthesized peptide.

Immunofluorescence analysis of rat-lung tissue. 1,Rho A Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of mouse-lung tissue. 1,Rho A Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunohistochemical analysis of paraffin-embedded Human-stomach-cancer tissue. 1,Rho A Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Rat-liver tissue. 1,Rho A Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,Rho A Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1,Rho A Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1,Rho A Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1,Rho A Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Western Blot analysis of various cells using Rho A Polyclonal Antibody diluted at 1：500