产品名称
RGS10 Rabbit Polyclonal Antibody
别名
RGS10; Regulator of G-protein signaling 10; RGS10
蛋白名称
Regulator of G-protein signaling 10
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=6001
Human Swissprot No.
O43665
Human Swissprot Link
http://www.uniprot.org/uniprotkb/O43665/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=67865
Mouse Swissprot No.
Q9CQE5
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q9CQE5
Rat Swissprot Link
http://www.uniprot.org/uniprot/P49806
免疫原
The antiserum was produced against synthesized peptide derived from human RGS10. AA range:80-129
特异性
RGS10 Polyclonal Antibody detects endogenous levels of RGS10 protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
Regulator of G protein signaling (RGS) family members are regulatory molecules that act as GTPase activating proteins (GAPs) for G alpha subunits of heterotrimeric G proteins. RGS proteins are able to deactivate G protein subunits of the Gi alpha, Go alpha and Gq alpha subtypes. They drive G proteins into their inactive GDP-bound forms. Regulator of G protein signaling 10 belongs to this family. All RGS proteins share a conserved 120-amino acid sequence termed the RGS domain. This protein associates specifically with the activated forms of the two related G-protein subunits, G-alphai3 and G-alphaz but fails to interact with the structurally and functionally distinct G-alpha subunits. Regulator of G protein signaling 10 protein is localized in the nucleus. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],
细胞定位
[Isoform 1]: Cytoplasm, cytosol . Nucleus . Forskolin treatment promotes phosphorylation and translocation to the nucleus. .; Nucleus .
功能
function:Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Associates specifically with the activated forms of the G protein subunits G(i)-alpha and G(z)-alpha but fails to interact with the structurally and functionally distinct G(s)-alpha subunit. Activity on G(z)-alpha is inhibited by palmitoylation of the G-protein.,PTM:Isoform 3 is phosphorylated on Ser-16.,similarity:Contains 1 RGS domain.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
