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RFX3 Rabbit Polyclonal Antibody

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产品基本信息

产品货号
BD-PT4066
产品名称
RFX3 Rabbit Polyclonal Antibody
别名
RFX3; Transcription factor RFX3; Regulatory factor X 3
类别
常规抗体
基因名称
RFX3
蛋白名称
Transcription factor RFX3
推荐应用
WB
反应种属
Human,Mouse
浓度
1 mg/ml
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene ID
5991
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=5991
Human Swissprot No.
P48380
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P48380/entry
Mouse Gene ID
19726
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=19726
Mouse Swissprot No.
P48381
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P48381
免疫原
The antiserum was produced against synthesized peptide derived from human RFX3. AA range:641-690
特异性
RFX3 Polyclonal Antibody detects endogenous levels of RFX3 protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200
预测分子量
84kD
运输及保存条件
-20°C/1 year
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene is a member of the regulatory factor X gene family, which encodes transcription factors that contain a highly-conserved winged helix DNA binding domain. The protein encoded by this gene is structurally related to regulatory factors X1, X2, X4, and X5. It is a transcriptional activator that can bind DNA as a monomer or as a heterodimer with other RFX family members. Multiple transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2013],
组织表达
Muscle,Testis,
细胞定位
Nucleus .
功能
function:Acts as a transcription factor. Represses transcription of MAP1A in non-neuronal cells but not in neuronal cells.,similarity:Belongs to the RFX family.,
期货
现货
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using RFX3 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from COLO and RAW264.7 cells, using RFX3 Antibody. The lane on the right is blocked with the synthesized peptide.

Western Blot analysis of various cells using RFX3 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).

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