产品名称
RFWD3 Rabbit Polyclonal Antibody
别名
RFWD3; RNF201; E3 ubiquitin-protein ligase RFWD3; RING finger and WD repeat domain-containing protein 3; RING finger protein 201
蛋白名称
E3 ubiquitin-protein ligase RFWD3
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=55159
Human Swissprot No.
Q6PCD5
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q6PCD5/entry
Mouse Swissprot No.
Q8CIK8
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q8CIK8
免疫原
The antiserum was produced against synthesized peptide derived from human RFWD3. AA range:374-423
特异性
RFWD3 Polyclonal Antibody detects endogenous levels of RFWD3 protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Contains 1 RING-type zinc finger.,similarity:Contains 3 WD repeats.,
组织表达
Brain,Teratocarcinoma,Testis,
细胞定位
Nucleus . Nucleus, PML body . Cytoplasm . In undamaged cells, found both in the cytoplasm and in the nucleus, partially associated with PML nuclear bodies (PubMed:21558276). In response to replication block, such as that caused by hydroxyurea treatment, or to DNA damage caused by ionizing radiations or doxorubicin, recruited to the nucleus, to stalled replication forks or to sites of DNA repair (PubMed:21504906, PubMed:28575657). This recruitment depends upon RPA2 (PubMed:21504906). .
功能
PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Contains 1 RING-type zinc finger.,similarity:Contains 3 WD repeats.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
