产品名称
RFC3 Rabbit Polyclonal Antibody
别名
RFC3; Replication factor C subunit 3; Activator 1 38 kDa subunit; A1 38 kDa subunit; Activator 1 subunit 3; Replication factor C 38 kDa subunit; RF-C 38 kDa subunit; RFC38
蛋白名称
Replication factor C subunit 3
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=5983
Human Swissprot No.
P40938
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P40938/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=69263
Mouse Swissprot No.
Q8R323
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q8R323
免疫原
The antiserum was produced against synthesized peptide derived from human RFC3. AA range:178-227
特异性
RFC3 Polyclonal Antibody detects endogenous levels of RFC3 protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kDa. This gene encodes the 38 kDa subunit. This subunit is essential for the interaction between the 140 kDa subunit and the core complex that consists of the 36, 37, and 40 kDa subunits. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008],
组织表达
Coronary artery,Placenta,
信号通路
DNA replication;Nucleotide excision repair;Mismatch repair;
功能
function:The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the activator 1 small subunits family.,subunit:Heterotetramer of subunits RFC2, RFC3, RFC4 and RFC5 that can form a complex either with RFC1 or with RAD17. The former interacts with PCNA in the presence of ATP, while the latter has ATPase activity but is not stimulated by PCNA.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
