产品名称
REPS1 Rabbit Polyclonal Antibody
别名
REPS1; RalBP1-associated Eps domain-containing protein 1; RalBP1-interacting protein 1
蛋白名称
RalBP1-associated Eps domain-containing protein 1
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=85021
Human Swissprot No.
Q96D71
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q96D71/entry
Mouse Swissprot No.
O54916
Mouse Swissprot Link
http://www.uniprot.org/uniprot/O54916
免疫原
The antiserum was produced against synthesized peptide derived from human REPS1. AA range:597-646
特异性
REPS1 Polyclonal Antibody detects endogenous levels of REPS1 protein.
稀释度
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a signaling adaptor protein with two EH domains that interacts with proteins that participate in signaling, endocytosis and cytoskeletal changes. The encoded protein has been found in association with intersectin 1 and Src homology 3-domain growth factor receptor-bound 2-like (endophilin) interacting protein 1 when intersectin 1 was isolated from clathrin-coated pits. The encoded protein has also been shown to interact with amphiphysin, a cytoplasmic protein at the surface of synaptic vesicles. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2014],
组织表达
Widely expressed with highest levels in heart and testis.
细胞定位
Membrane, clathrin-coated pit . Colocalize with ITSN1 at the plasma membrane in structures that are most probably clathrin-coated pits.
功能
function:May coordinate the cellular actions of activated EGF receptors and Ral-GTPases.,PTM:EGF stimulates phosphorylation on Tyr-residues.,similarity:Contains 1 EF-hand domain.,similarity:Contains 1 EH domain.,subunit:Homodimer (Potential). Interacts with RAB11FIP2. Interacts with RALBP1, CRK and GRB2. Binding to RALBP1 does not affect its Ral-binding activity. Forms a complex with the SH3 domains of CRK and GRB2 which may link it to an EGF-responsive tyrosine kinase.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
