产品名称
RECK Rabbit Polyclonal Antibody
别名
RECK; ST15; Reversion-inducing cysteine-rich protein with Kazal motifs; hRECK; Suppressor of tumorigenicity 15 protein
蛋白名称
Reversion-inducing cysteine-rich protein with Kazal motifs
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=8434
Human Swissprot No.
O95980
Human Swissprot Link
http://www.uniprot.org/uniprotkb/O95980/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=53614
Mouse Swissprot No.
Q9Z0J1
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q9Z0J1
免疫原
The antiserum was produced against synthesized peptide derived from human RECK. AA range:21-70
特异性
RECK Polyclonal Antibody detects endogenous levels of RECK protein.
稀释度
WB 1:500-2000 IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
The protein encoded by this gene is a cysteine-rich, extracellular protein with protease inhibitor-like domains whose expression is suppressed strongly in many tumors and cells transformed by various kinds of oncogenes. In normal cells, this membrane-anchored glycoprotein may serve as a negative regulator for matrix metalloproteinase-9, a key enzyme involved in tumor invasion and metastasis. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2015],
组织表达
Expressed in various tissues and untransformed cells (PubMed:9789069). It is undetectable in tumor-derived cell lines and oncogenically transformed cells (PubMed:9789069).
细胞定位
Cell membrane ; Lipid-anchor, GPI-anchor .
功能
function:Negatively regulates matrix metalloproteinase-9 (MMP-9) by suppressing MMP-9 secretion and by direct inhibition of its enzymatic activity. RECK down-regulation by oncogenic signals may facilitate tumor invasion and metastasis. Appears to also regulate MMP-2 and MT1-MMP, which are involved in cancer progression.,PTM:N-glycosylated.,similarity:Contains 3 Kazal-like domains.,subunit:Interacts with MMP-9.,tissue specificity:Expressed in various tissues and untransformed cells. It is undetectable in tumor-derived cell lines and oncogenically transformed cells.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
