产品名称
RCC2 Rabbit Polyclonal Antibody
别名
RCC2; KIAA1470; TD60; Protein RCC2; RCC1-like protein TD-60; Telophase disk protein of 60 kDa
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=55920
Human Swissprot No.
Q9P258
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q9P258/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=108911
Mouse Swissprot No.
Q8BK67
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q8BK67
免疫原
The antiserum was produced against synthesized peptide derived from human RCC2. AA range:473-522
特异性
RCC2 Polyclonal Antibody detects endogenous levels of RCC2 protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
function:Required for completion of mitosis and cytokinesis. May function as a guanine nucleotide exchange factor for the small GTPase RAC1.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Contains 7 RCC1 repeats.,subcellular location:Appears in the nucleus at G2, then concentrates at the inner centromere region of chromosomes during prophase. Redistributes to the midzone of the mitotic spindle during anaphase. Here, the protein covers the entire equatorial diameter from cortex to cortex.,subunit:Binds preferentially to the nucleotide-free form of RAC1. Interacts with microtubules.,
组织表达
Brain,Epithelium,Melanoma,Muscle,Natural killer cell,Uterus,
细胞定位
Nucleus, nucleolus . Nucleus . Cytoplasm, cytoskeleton . Chromosome, centromere . Cytoplasm, cytoskeleton, spindle . Chromosome . Midbody . Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Appears in the nucleus at G2, then concentrates at the inner centromere region of chromosomes during prophase. Redistributes to the midzone of the mitotic spindle during anaphase. Here, the protein covers the entire equatorial diameter from cortex to cortex (PubMed:12919680, PubMed:1939370, PubMed:7559776, PubMed:9914378). Colocalizes with cytoplasmic microtubules in interphase cells (PubMed:23388455). Colocalizes with RAC1 at the cell membrane (PubMed:25074804). .
功能
function:Required for completion of mitosis and cytokinesis. May function as a guanine nucleotide exchange factor for the small GTPase RAC1.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Contains 7 RCC1 repeats.,subcellular location:Appears in the nucleus at G2, then concentrates at the inner centromere region of chromosomes during prophase. Redistributes to the midzone of the mitotic spindle during anaphase. Here, the protein covers the entire equatorial diameter from cortex to cortex.,subunit:Binds preferentially to the nucleotide-free form of RAC1. Interacts with microtubules.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
