产品名称
RARα Rabbit Polyclonal Antibody
别名
RARA; NR1B1; Retinoic acid receptor alpha; RAR-alpha; Nuclear receptor subfamily 1 group B member 1
蛋白名称
Retinoic acid receptor alpha
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=5914
Human Swissprot No.
P10276
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P10276/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=19401
Mouse Swissprot No.
P11416
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P11416
免疫原
The antiserum was produced against synthesized peptide derived from human Retinoic Acid Receptor alpha. AA range:46-95
特异性
RARα Polyclonal Antibody detects endogenous levels of RARα protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene represents a nuclear retinoic acid receptor. The encoded protein, retinoic acid receptor alpha, regulates transcription in a ligand-dependent manner. This gene has been implicated in regulation of development, differentiation, apoptosis, granulopoeisis, and transcription of clock genes. Translocations between this locus and several other loci have been associated with acute promyelocytic leukemia. Alternatively spliced transcript variants have been found for this locus.[provided by RefSeq, Sep 2010],
组织表达
Expressed in monocytes.
细胞定位
Nucleus . Cytoplasm . Nuclear localization depends on ligand binding, phosphorylation and sumoylation (PubMed:19850744). Translocation to the nucleus in the absence of ligand is dependent on activation of PKC and the downstream MAPK phosphorylation (By similarity). Increased nuclear localization upon pulsatile shear stress (PubMed:28167758). .
信号通路
Pathways in cancer;Acute myeloid leukemia;
功能
disease:Chromosomal aberrations involving RARA may be a cause of acute promyelocytic leukemia (APL) [MIM:612376]. Translocation t(11;17)(q32;q21) with ZBTB16/PLZF; translocation t(15;17)(q21;q21) with PML; translocation t(5;17)(q32;q11) with NPM.,domain:Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal steroid-binding domain.,function:This is a receptor for retinoic acid. This metabolite has profound effects on vertebrate development. Retinoic acid is a morphogen and is a powerful teratogen. This receptor controls cell function by directly regulating gene expression.,online information:Retinoic acid receptor entry,PTM:Phosphorylated. Phosphorylation does not change during cell cycle. Phosphorylation on Ser-77 is crucial for transcriptional activity.,similarity:Belongs to the nuclear hormone receptor family.,similarity:Belongs to the nuclear hormone receptor family. NR1 subfamily.,similarity:Contains 1 nuclear receptor DNA-binding domain.,subunit:Interacts with CDK7 (By similarity). Interacts with NCOA3 and NCOA6 coactivators, leading to a strong increase of transcription of target genes. Interacts with NOCA7 in a ligand-inducible manner.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
