BD-PT3969-Rad52 Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

Rad52 Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PT3969

产品名称

Rad52 Rabbit Polyclonal Antibody

别名

RAD52; DNA repair protein RAD52 homolog

类别

常规抗体

基因名称

RAD52

蛋白名称

DNA repair protein RAD52 homolog

推荐应用

反应种属

Human,Mouse

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

5893

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=5893

Human Swissprot No.

P43351

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P43351/entry

Mouse Gene ID

19365

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=19365

Mouse Swissprot No.

P43352

Mouse Swissprot Link

http://www.uniprot.org/uniprot/P43352

免疫原

The antiserum was produced against synthesized peptide derived from human RAD52. AA range:70-119

特异性

Rad52 Polyclonal Antibody detects endogenous levels of Rad52 protein.

稀释度

WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

预测分子量

46kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

The protein encoded by this gene shares similarity with Saccharomyces cerevisiae Rad52, a protein important for DNA double-strand break repair and homologous recombination. This gene product was shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombination protein RAD51, which suggested its role in RAD51 related DNA recombination and repair. A pseudogene of this gene is present on chromosome 2. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Jul 2014],

组织表达

Amygdala,Brain,Embryonal rhabdomyosarcoma,Hippocampus,PCR rescued c

细胞定位

Nucleus .

信号通路

Homologous recombination;

功能

function:Involved in double-stranded break repair. Plays a central role in genetic recombination and DNA repair by promoting the annealing of complementary single-stranded DNA and by stimulation of the RAD51 recombinase.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the RAD52 family.,subunit:Forms a undecameric ring.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunofluorescence analysis of COS7 cells, using RAD52 Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human testis tissue, using RAD52 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from 293 cells, using RAD52 Antibody. The lane on the right is blocked with the synthesized peptide.

Western Blot analysis of various cells using Rad52 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit （SC-003,Inventbiotech,MN,USA）.

Western Blot analysis of 293 cells using Rad52 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit （SC-003,Inventbiotech,MN,USA）.