产品名称
Rad23B Rabbit Polyclonal Antibody
别名
RAD23B; UV excision repair protein RAD23 homolog B; HR23B; hHR23B; XP-C repair-complementing complex 58 kDa protein; p58
蛋白名称
UV excision repair protein RAD23 homolog B
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=5887
Human Swissprot No.
P54727
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P54727/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=19359
Mouse Swissprot No.
P54728
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P54728
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=298012
Rat Swissprot Link
http://www.uniprot.org/uniprot/Q4KMA2
免疫原
The antiserum was produced against synthesized peptide derived from human RAD23B. AA range:1-50
特异性
Rad23B Polyclonal Antibody detects endogenous levels of Rad23B protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
The protein encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in the nucleotide excision repair (NER). This protein was found to be a component of the protein complex that specifically complements the NER defect of xeroderma pigmentosum group C (XP-c) cell extracts in vitro. This protein was also shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, and thus this protein may be involved in the ubiquitin mediated proteolytic pathway in cells. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Sep 2011],
组织表达
Epithelium,Testis,Thymus,Uterus,
细胞定位
Nucleus. Cytoplasm. The intracellular distribution is cell cycle dependent. Localized to the nucleus and the cytoplasm during G1 phase. Nuclear levels decrease during S-phase; upon entering mitosis, relocalizes in the cytoplasm without association with chromatin.
信号通路
Nucleotide excision repair;
功能
domain:The ubiquitin-like domain mediates interaction with MJD.,function:Plays a central role both in proteosomal degradation of misfolded proteins and DNA repair. Central component of a complex required to couple deglycosylation and proteasome-mediated degradation of misfolded proteins in the endoplasmic reticulun that are retrotranslocated in the cytosol. Involved in DNA excision repair by stabilizing XPC protein. May play a part in DNA damage recognition and/or in altering chromatin structure to allow access by damage-processing enzymes.,similarity:Belongs to the RAD23 family.,similarity:Contains 1 STI1 domain.,similarity:Contains 1 ubiquitin-like domain.,similarity:Contains 2 UBA domains.,subunit:Component of a complex required to couple retrotranslocation, ubiquitination and deglycosylation composed of NGLY1, SAKS1, AMFR, VCP and RAD23B (By similarity). Interacts with the 26S proteasome. Interacts directly with NGLY1. Heterodimer of a 125 kDa subunit (p125) and of a 58 kDa subunit (p58). Interacts with MJD and XPC.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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