产品名称
PU.1 Rabbit Polyclonal Antibody
别名
SPI1; Transcription factor PU.1; 31 kDa-transforming protein
蛋白名称
Transcription factor PU.1
反应种属
Human,Mouse,Rat,Monkey
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=6688
Human Swissprot No.
P17947
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P17947/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=20375
Mouse Swissprot No.
P17433
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P17433
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=366126
Rat Swissprot Link
http://www.uniprot.org/uniprot/Q6BDS1
免疫原
The antiserum was produced against synthesized peptide derived from human SPI1. AA range:181-230
特异性
PU.1 Polyclonal Antibody detects endogenous levels of PU.1 protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],
信号通路
Pathways in cancer;Acute myeloid leukemia;
功能
function:Binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. Also binds RNA and may modulate pre-mRNA splicing.,induction:Highly expressed in both FV-P and FV-A-induced erythro-leukemia cell lines that have undergone rearrangements of the Spi-1 gene due to the insertion of SFFV.,similarity:Belongs to the ETS family.,similarity:Contains 1 ETS DNA-binding domain.,subunit:Binds DNA as a monomer. Interacts with RUNX1 and SPIB. Interacts with CEBPD and NONO.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
