产品名称
PTP1B Rabbit Polyclonal Antibody
别名
PTPN1; PTP1B; Tyrosine-protein phosphatase non-receptor type 1; Protein-tyrosine phosphatase 1B; PTP-1B
蛋白名称
Tyrosine-protein phosphatase non-receptor type 1
反应种属
Human,Mouse,Rat,Monkey
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=5770
Human Swissprot No.
P18031
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P18031/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=19246
Mouse Swissprot No.
P35821
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P35821
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=24697
Rat Swissprot Link
http://www.uniprot.org/uniprot/P20417
免疫原
The antiserum was produced against synthesized peptide derived from human PTP1B. AA range:16-65
特异性
PTP1B Polyclonal Antibody detects endogenous levels of PTP1B protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
The protein encoded by this gene is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of
组织表达
Expressed in keratinocytes (at protein level).
细胞定位
Endoplasmic reticulum membrane ; Peripheral membrane protein ; Cytoplasmic side . Interacts with EPHA3 at the cell membrane.
信号通路
Adherens_Junction;Insulin_Receptor;
功能
catalytic activity:Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate.,function:May play an important role in CKII- and p60c-src-induced signal transduction cascades.,PTM:Oxidized on Cys-215; the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a 4-amino-3-isothiazolidinone serine cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction.,similarity:Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily.,similarity:Contains 1 tyrosine-protein phosphatase domain.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
