产品名称
PRIM1 Rabbit Polyclonal Antibody
别名
PRIM1; DNA primase small subunit; DNA primase 49 kDa subunit; p49
蛋白名称
DNA primase small subunit
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=5557
Human Swissprot No.
P49642
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P49642/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=19075
Mouse Swissprot No.
P20664
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P20664
免疫原
The antiserum was produced against synthesized peptide derived from human PRIM1. AA range:371-420
特异性
PRIM1 Polyclonal Antibody detects endogenous levels of PRIM1 protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
The replication of DNA in eukaryotic cells is carried out by a complex chromosomal replication apparatus, in which DNA polymerase alpha and primase are two key enzymatic components. Primase, which is a heterodimer of a small subunit and a large subunit, synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. The protein encoded by this gene is the small, 49 kDa primase subunit. [provided by RefSeq, Jul 2008],
细胞定位
nucleoplasm,alpha DNA polymerase:primase complex,membrane,primosome complex,
信号通路
Purine metabolism;Pyrimidine metabolism;DNA replication;
功能
function:DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication.,miscellaneous:The bound zinc ion is not a cofactor. It is bound to a zinc knuckle motif that may be involved in sequence recognition and the binding of ssDNA.,similarity:Belongs to the eukaryotic-type primase small subunit family.,subunit:Heterodimer of a small subunit and a large subunit.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
