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PRAF2 Rabbit Polyclonal Antibody

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产品基本信息

产品货号
BD-PT3847
产品名称
PRAF2 Rabbit Polyclonal Antibody
别名
PRAF2; JM4; PRA1 family protein 2
类别
常规抗体
基因名称
PRAF2
蛋白名称
PRA1 family protein 2
推荐应用
WB
反应种属
Human,Mouse,Rat
浓度
1 mg/ml
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene ID
11230
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=11230
Human Swissprot No.
O60831
Human Swissprot Link
http://www.uniprot.org/uniprotkb/O60831/entry
Mouse Gene ID
54637
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=54637
Mouse Swissprot No.
Q9JIG8
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q9JIG8
免疫原
The antiserum was produced against synthesized peptide derived from human JM4. AA range:129-178
特异性
PRAF2 Polyclonal Antibody detects endogenous levels of PRAF2 protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.
预测分子量
20kD
运输及保存条件
-20°C/1 year
宿主
Polyclonal, Rabbit,IgG
背景介绍
function:May be involved in ER/Golgi transport and vesicular traffic. Plays a proapoptic role in cerulenin-induced neuroblastoma apoptosis.,similarity:Belongs to the PRA1 family.,subunit:Interacts with CCR5 and GDE1.,tissue specificity:Strong expression in the brain, small intestine, lung, spleen, and pancreas as well as in tumor tissues of the breast, colon, lung and ovary, with a weaker expression in normal tissues of the same patient. High expression in neuroblastic tumors. Strongly expressed in Purkinje cells and more moderately in cells of the molecular and the granular layers in the cerebellum. Detected in neuronal cells, but not in non-neuronal cells in the cerebral cortex, hippocampus, and lateral ventricles.,
组织表达
Strong expression in the brain, small intestine, lung, spleen, and pancreas as well as in tumor tissues of the breast, colon, lung and ovary, with a weaker expression in normal tissues of the same patient. High expression in neuroblastic tumors. Strongly expressed in Purkinje cells and more moderately in cells of the molecular and the granular layers in the cerebellum. Detected in neuronal cells, but not in non-neuronal cells in the cerebral cortex, hippocampus, and lateral ventricles.
细胞定位
Endosome membrane ; Multi-pass membrane protein .
功能
function:May be involved in ER/Golgi transport and vesicular traffic. Plays a proapoptic role in cerulenin-induced neuroblastoma apoptosis.,similarity:Belongs to the PRA1 family.,subunit:Interacts with CCR5 and GDE1.,tissue specificity:Strong expression in the brain, small intestine, lung, spleen, and pancreas as well as in tumor tissues of the breast, colon, lung and ovary, with a weaker expression in normal tissues of the same patient. High expression in neuroblastic tumors. Strongly expressed in Purkinje cells and more moderately in cells of the molecular and the granular layers in the cerebellum. Detected in neuronal cells, but not in non-neuronal cells in the cerebral cortex, hippocampus, and lateral ventricles.,
期货
现货
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using JM4 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from LOVO cells, using JM4 Antibody. The lane on the right is blocked with the synthesized peptide.

Western Blot analysis of various cells using PRAF2 Polyclonal Antibody diluted at 1:2000

Western Blot analysis of RAT-HEART cells using PRAF2 Polyclonal Antibody diluted at 1:2000

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