BD-PT3835-PPAR-α Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

PPAR-α Rabbit Polyclonal Antibody

产品详情相关文献产品问答相关产品

产品基本信息

产品货号

BD-PT3835

产品名称

PPAR-α Rabbit Polyclonal Antibody

别名

PPARA; NR1C1; PPAR; Peroxisome proliferator-activated receptor alpha; PPAR-alpha; Nuclear receptor subfamily 1 group C member 1

类别

常规抗体

基因名称

PPARA

蛋白名称

Peroxisome proliferator-activated receptor alpha

推荐应用

反应种属

Human,Mouse,Rat

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

5465

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=5465

Human Swissprot No.

Q07869

Human Swissprot Link

http://www.uniprot.org/uniprotkb/Q07869/entry

Mouse Gene ID

19013

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=19013

Mouse Swissprot No.

P23204

Mouse Swissprot Link

http://www.uniprot.org/uniprot/P23204

Rat Gene ID

25747

Rat Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=25747

Rat Swissprot No.

P37230

Rat Swissprot Link

http://www.uniprot.org/uniprot/P37230

免疫原

The antiserum was produced against synthesized peptide derived from human PPAR-alpha. AA range:6-55

特异性

PPAR-α Polyclonal Antibody detects endogenous levels of PPAR-α protein.

稀释度

WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300. IF 1:50-200

预测分子量

52kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

peroxisome proliferator activated receptor alpha(PPARA) Homo sapiens Peroxisome proliferators include hypolipidemic drugs, herbicides, leukotriene antagonists, and plasticizers; this term arises because they induce an increase in the size and number of peroxisomes. Peroxisomes are subcellular organelles found in plants and animals that contain enzymes for respiration and for cholesterol and lipid metabolism. The action of peroxisome proliferators is thought to be mediated via specific receptors, called PPARs, which belong to the steroid hormone receptor superfamily. PPARs affect the expression of target genes involved in cell proliferation, cell differentiation and in immune and inflammation responses. Three closely related subtypes (alpha, beta/delta, and gamma) have been identified. This gene encodes the subtype PPAR-alpha, which is a nuclear transcription factor. Multiple alternatively spliced transcript variants have been described for thi

组织表达

Skeletal muscle, liver, heart and kidney. Expressed in monocytes (PubMed:28167758).

细胞定位

Nucleus.

信号通路

PPAR;Adipocytokine;

功能

function:Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the receptor binds to a promoter element in the gene for acyl-CoA oxidase and activates its transcription. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids.,online information:Peroxisome proliferator-activated receptor entry,similarity:Belongs to the nuclear hormone receptor family. NR1 subfamily.,similarity:Contains 1 nuclear receptor DNA-binding domain.,subunit:Heterodimer with the retinoid X receptor. Interacts with NCOA3 and NCOA6 coactivators, leading to a strong increase of transcription of target genes. Also interacts with PPARBP coactivator in vitro. Interacts with AKAP13.,tissue specificity:Skeletal muscle, liver, heart and kidney.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunofluorescence analysis of HeLa cells, using PPAR-alpha Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from NIH/3T3 cells, using PPAR-alpha Antibody. The lane on the right is blocked with the synthesized peptide.

Immunofluorescence analysis of rat-lung tissue. 1,PPAR-α Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of rat-spleen tissue. 1,PPAR-α Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of mouse-kidney tissue. 1,PPAR-α Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. 1,PPAR-α Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1,PPAR-α Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Mouse-spleen tissue. 1,PPAR-α Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Western Blot analysis of 3T3 cells using PPAR-α Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit （SC-003,Inventbiotech,MN,USA）.