产品名称
PP4R2 Rabbit Polyclonal Antibody
别名
PPP4R2; SBBI57; Serine/threonine-protein phosphatase 4 regulatory subunit 2
蛋白名称
Serine/threonine-protein phosphatase 4 regulatory subunit 2
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=151987
Human Swissprot No.
Q9NY27
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q9NY27/entry
Mouse Swissprot No.
Q0VGB7
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q0VGB7
免疫原
The antiserum was produced against synthesized peptide derived from human PPP4R2. AA range:171-220
特异性
PP4R2 Polyclonal Antibody detects endogenous levels of PP4R2 protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
The protein encoded by this gene is a regulatory subunit of the serine/threonine-protein phosphatase 4 complex. In addition to being required for efficient DNA double strand break repair, this complex plays a role in organization of microtubules at centrosomes and processing of spliceosomal snRNPs. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2015],
细胞定位
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Nucleus. Ionizing radiation induces relocalization to nuclear foci and colocalization with RPA2.
功能
function:Regulatory subunit of serine/threonine-protein phosphatase 4 (PP4). May regulate the activity of PPP4C at centrosomal microtubule organizing centers. Its interaction with the SMN complex leads to enhance the temporal localization of snRNPs, suggesting a role of PPP4C in maturation of spliceosomal snRNPs. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on 'Ser-140' (gamma-H2AFX) generated during DNA replication and required for DNA DSB repair.,sequence caution:Contaminating sequence. Potential poly-A sequence.,similarity:Belongs to the PPP4R2 family.,subunit:Serine/threonine-protein phosphatase 4 (PP4) occurs in different assemblies of the catalytic and one or more regulatory subunits. Component of the PP4 complexes PPP4C-PPP4R2, PPP4C-PPP4R2-PPP4R3A and PPP4C-PPP4R2-PPP4R3B. The PPP4C-PPP4R2 complex appears to be a tetramer composed of 2 molecules of PPP4C and 2 molecules of PPP4R2. Interacts with DDX20/GEMIN3 and GEMIN4.,tissue specificity:Widely expressed.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
