BD-PT3817-POLR3E Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

POLR3E Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PT3817

产品名称

POLR3E Rabbit Polyclonal Antibody

别名

POLR3E; KIAA1452; DNA-directed RNA polymerase III subunit RPC5; RNA polymerase III subunit C5; DNA-directed RNA polymerase III 80 kDa polypeptide

类别

常规抗体

基因名称

POLR3E

蛋白名称

DNA-directed RNA polymerase III subunit RPC5

推荐应用

反应种属

Human,Mouse

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

55718

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=55718

Human Swissprot No.

Q9NVU0

Human Swissprot Link

http://www.uniprot.org/uniprotkb/Q9NVU0/entry

Mouse Gene ID

26939

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=26939

Mouse Swissprot No.

Q9CZT4

Mouse Swissprot Link

http://www.uniprot.org/uniprot/Q9CZT4

免疫原

The antiserum was produced against synthesized peptide derived from human RPC5. AA range:191-240

特异性

POLR3E Polyclonal Antibody detects endogenous levels of POLR3E protein.

稀释度

WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.

预测分子量

80kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

function:DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Essential for efficient transcription from both the type 2 VAI and type 3 U6 RNA polymerase III promoters.,subunit:Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits (By similarity). Interacts with POLR3D/RPC4.,

组织表达

Brain,Eye,Liver,Teratocarcinoma,

细胞定位

Nucleus.

功能

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunofluorescence analysis of LOVO cells, using RPC5 Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using RPC5 Antibody. The picture on the right is blocked with the synthesized peptide.

Western Blot analysis of various cells using POLR3E Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit （SC-003,Inventbiotech,MN,USA）.

Western Blot analysis of JK cells using POLR3E Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit （SC-003,Inventbiotech,MN,USA）.

Immunohistochemical analysis of paraffin-embedded Human colon cancer. Antibody was diluted at 1:100（4°,overnight）. High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl （right） obtaned from antibody was pre-absorbed by immunogen peptide.