产品名称
PNPase Rabbit Polyclonal Antibody
别名
PNPT1; PNPASE; Polyribonucleotide nucleotidyltransferase 1; mitochondrial; 3'-5' RNA exonuclease OLD35; PNPase old-35; Polynucleotide phosphorylase 1; PNPase 1; Polynucleotide phosphorylase-like protein
蛋白名称
Polyribonucleotide nucleotidyltransferase 1 mitochondrial
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=87178
Human Swissprot No.
Q8TCS8
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q8TCS8/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=71701
Mouse Swissprot No.
Q8K1R3
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q8K1R3
免疫原
The antiserum was produced against synthesized peptide derived from human PNPT1. AA range:570-619
特异性
PNPase Polyclonal Antibody detects endogenous levels of PNPase protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
The protein encoded by this gene belongs to the evolutionary conserved polynucleotide phosphorylase family comprised of phosphate dependent 3'-to-5' exoribonucleases implicated in RNA processing and degradation. This enzyme is predominantly localized in the mitochondrial intermembrane space and is involved in import of RNA to mitochondria. Mutations in this gene have been associated with combined oxidative phosphorylation deficiency-13 and autosomal recessive nonsyndromic deafness-70. Related pseudogenes are found on chromosomes 3 and 7. [provided by RefSeq, Dec 2012],
组织表达
Cervix,Epithelium,Melanoma,Skin,Teratocarcinoma,Urinary bla
细胞定位
Cytoplasm . Mitochondrion matrix . Mitochondrion intermembrane space ; Peripheral membrane protein .
信号通路
Purine metabolism;Pyrimidine metabolism;RNA degradation;
功能
catalytic activity:RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.,function:Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction.,induction:Up-regulated in cells upon senescence and terminal differentiation. Up-regulated after treatment with interferon beta (IFN-beta).,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the polyribonucleotide nucleotidyltransferase family.,similarity:Contains 1 KH domain.,similarity:Contains 1 S1 motif domain.,subunit:Homotrimer (Potential). Interacts with TCL1A; the interaction has no effect on PNPT1 exonuclease activity.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
