产品名称
PKD2 Rabbit Polyclonal Antibody
别名
PRKD2; PKD2; HSPC187; Serine/threonine-protein kinase D2; nPKC-D2
蛋白名称
Serine/threonine-protein kinase D2
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=25865
Human Swissprot No.
Q9BZL6
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q9BZL6/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=101540
Mouse Swissprot No.
Q8BZ03
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q8BZ03
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=292658
Rat Swissprot Link
http://www.uniprot.org/uniprot/Q5XIS9
免疫原
The antiserum was produced against synthesized peptide derived from human PKD2. AA range:829-878
特异性
PKD2 Polyclonal Antibody detects endogenous levels of PKD2 protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
The protein encoded by this gene belongs to the protein kinase D (PKD) family of serine/threonine protein kinases. This kinase can be activated by phorbol esters as well as by gastrin via the cholecystokinin B receptor (CCKBR) in gastric cancer cells. It can bind to diacylglycerol (DAG) in the trans-Golgi network (TGN) and may regulate basolateral membrane protein exit from TGN. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008],
细胞定位
Cytoplasm . Cell membrane . Nucleus . Golgi apparatus, trans-Golgi network . Translocation to the cell membrane is required for kinase activation. Accumulates in the nucleus upon CK1-mediated phosphorylation after activation of G-protein-coupled receptors. Nuclear accumulation is regulated by blocking nuclear export of active PRKD2 rather than by increasing import. .
信号通路
Regulation_Microtubule; Regulation of Actin Dynamics; Stem cell pathway; Insulin Receptor; B Cell Receptor; AMPK
功能
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,enzyme regulation:Activated by diacylglycerol and phorbol esters.,function:Calcium-independent, phospholipid-dependent, serine- and threonine-specific protein kinase.,PTM:Autophosphorylated. Phorbol esters stimulates autophosphorylation. Phosphorylation of Ser-876 correlates with the activation status of the kinase.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.,similarity:Contains 1 PH domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 2 phorbol-ester/DAG-type zinc fingers.,tissue specificity:Widely expressed.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
