产品名称
PKD1 Rabbit Polyclonal Antibody
别名
PRKD1; PKD; PKD1; PRKCM; Serine/threonine-protein kinase D1; Protein kinase C mu type; Protein kinase D; nPKC-D1; nPKC-mu
蛋白名称
Serine/threonine-protein kinase D1
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=5587
Human Swissprot No.
Q15139
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q15139/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=18760
Mouse Swissprot No.
Q62101
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q62101
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=85421
Rat Swissprot Link
http://www.uniprot.org/uniprot/Q9WTQ1
免疫原
The antiserum was produced against synthesized peptide derived from human PKD1/PKC mu. AA range:171-220
特异性
PKD1 Polyclonal Antibody detects endogenous levels of PKD1 protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
PRKD1 is a serine/threonine kinase that regulates a variety of cellular functions, including membrane receptor signaling, transport at the Golgi, protection from oxidative stress at the mitochondria, gene transcription, and regulation of cell shape, motility, and adhesion (summary by Eiseler et al., 2009 [PubMed 19329994]).[supplied by OMIM, Nov 2010],
细胞定位
Cytoplasm . Cell membrane . Golgi apparatus, trans-Golgi network . Translocation to the cell membrane is required for kinase activation.
信号通路
Regulation_Microtubule; Regulation of Actin Dynamics; Stem cell pathway; Insulin Receptor; B Cell Receptor; AMPK
功能
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,enzyme regulation:Activated by diacylglycerol and phorbol esters.,function:Calcium-independent, phospholipid-dependent, serine- and threonine-specific kinase involved in resistance to oxidative stress.,PTM:Phosphorylation of Ser-738 and/or Ser-742 in activated PKD is mediated by transphosphorylation (By similarity). Phosphorylation of Tyr-463 mediated by the Src/Abl pathway in response to oxidative stress activates the kinase.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.,similarity:Contains 1 PH domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 2 phorbol-ester/DAG-type zinc fingers.,subunit:Interacts (via N-terminus) with ADAP1/CENTA1. Interacts with Src.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
