BD-PT3767

PKC θ Rabbit Polyclonal Antibody

PRKCQ; PRKCT; Protein kinase C theta type; nPKC-theta

常规抗体

PRKCQ

Protein kinase C theta type

WB

Human,Mouse,Rat

1 mg/ml

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

5588

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=5588

Q04759

http://www.uniprot.org/uniprotkb/Q04759/entry

18761

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=18761

Q02111

http://www.uniprot.org/uniprot/Q02111

Q9WTQ0

http://www.uniprot.org/uniprot/Q9WTQ0

The antiserum was produced against synthesized peptide derived from human PKC thet. AA range:504-553

PKC θ Polyclonal Antibody detects endogenous levels of PKC θ protein.

WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200

78kD

-20°C/1 year

Polyclonal, Rabbit,IgG

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipid-dependent protein kinase. This kinase is important for T-cell activation. It is required for the activation of the transcription factors NF-kappaB and AP-1, and may link the T cell receptor (TCR) signaling complex to the activation of the transcription factors. [provided by RefSeq, Jul 2008],

Expressed in skeletal muscle, T-cells, megakaryoblastic cells and platelets.

Cytoplasm. Cell membrane; Peripheral membrane protein. In resting T-cells, mostly localized in cytoplasm. In response to TCR stimulation, associates with lipid rafts and then localizes in the immunological synapse.

Regulation_Microtubule; Regulation of Actin Dynamics; Stem cell pathway; Insulin Receptor; NF_kappaB; B Cell Receptor; AMPK

catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,domain:The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.,enzyme regulation:Three specific sites; Thr-538 (activation loop of the kinase domain), Ser-676 (turn motif) and Ser-695 (hydrophobic region), need to be phosphorylated for its full activation.,function:PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.,function:This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production.,PTM:Autophosphorylation at Thr-219 is required for targeting to the TCR and cellular function of PKC upon antigen receptor ligation.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.,similarity:Contains 1 AGC-kinase C-terminal domain.,similarity:Contains 1 C2 domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 2 phorbol-ester/DAG-type zinc fingers.,subunit:Interacts with TXNL2/PICOT.,tissue specificity:Skeletal muscle, megakaryoblastic cells and platelets.,

现货

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.