BD-PT3763

PKC ε Rabbit Polyclonal Antibody

PRKCE; PKCE; Protein kinase C epsilon type; nPKC-epsilon

常规抗体

PRKCE

Protein kinase C epsilon type

WB

Human,Mouse,Rat

1 mg/ml

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

5581

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=5581

Q02156

http://www.uniprot.org/uniprotkb/Q02156/entry

18754

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=18754

P16054

http://www.uniprot.org/uniprot/P16054

29340

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=29340

P09216

http://www.uniprot.org/uniprot/P09216

The antiserum was produced against synthesized peptide derived from human PKC epsilon. AA range:688-737

PKC ε Polyclonal Antibody detects endogenous levels of PKC ε protein.

WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000

83kD

-20°C/1 year

Polyclonal, Rabbit,IgG

protein kinase C epsilon(PRKCE) Homo sapiens Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been shown to be involved in many different cellular functions, such as neuron channel activation, apoptosis, cardioprotection from ischemia, heat shock response, as well as insulin exocytosis. Knockout studies in mice suggest that this kinase is important for lipopolysaccharide (LPS)-mediated signaling in activated macro

Expressed in cumulus cells (at protein level).

Cytoplasm . Cytoplasm, cytoskeleton . Cell membrane . Cytoplasm, perinuclear region . Nucleus . Translocated to plasma membrane in epithelial cells stimulated by HGF (PubMed:17603037). Associated with the Golgi at the perinuclear site in pre-passage fibroblasts (By similarity). In passaging cells, translocated to the cell periphery (By similarity). Translocated to the nucleus in PMA-treated cells (By similarity). .

Regulation_Microtubule; Regulation of Actin Dynamics; Stem cell pathway; Insulin Receptor; B Cell Receptor; AMPK

catalytic activity:ATP + a protein = ADP + a phosphoprotein.,domain:The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.,enzyme regulation:Three specific sites; Thr-566 (activation loop of the kinase domain), Thr-710 (turn motif) and Ser-729 (hydrophobic region), need to be phosphorylated for its full activation.,function:PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.,function:This is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme.,PTM:Phosphorylation on Thr-566 by PDPK1 triggers autophosphorylation on Ser-729.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.,similarity:Contains 1 AGC-kinase C-terminal domain.,similarity:Contains 1 C2 domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 2 phorbol-ester/DAG-type zinc fingers.,subunit:Forms a ternary complex with TRIM63 and GN2BL1.,

现货

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.